权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Research in small scale screening methods for the hazard assessment of the toxic substances in aquatic environment

水环境有毒物质危害评估小规模筛查方法研究

基本信息

批准号：
06680522
负责人：
KUSUI Takashi
金额：
$ 1.34万
依托单位：
College of Technology, Toyama Prefectural University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06680522/
关键词：
bioassay toxicity mutagenicity hazard assessment microplate industrial wastewater umu test screening bicassay hazard

项目摘要

(1) The way to diminish the effect of volatile sample on themicroplate-based algal assay was examined. The uses of ceiling tape and Teflon sheet, which prevented the contamination from the adjacent wells, were found to not only diminish the effect of volatile samples, but also lower the cell yield compared to that of usual method. These operations did not affect the IC50 (concentration of 50% inhibition) significantly, suggesting that these techniques would improve the performance of microplate method.(2) To enhance the efficiency of umu test, the 96-wells microplate-based method was developed. The major modifications from original methods are as follows, volume of reagents and bacterial culture, incubation temperature, concentration of SDS,wave length for absorbance measurement. Finally the modified Miller's equation was conducted to calculate the unit activity of beta galactocidase in microplate assay,(3) The sensitivity of small scale hydra acute toxicity test based on the morphological change was examined. The values of 96h-EC50 showed the relatively higher sensitivity to Cr6+than other assays examined. For some metals, the effect was transient, showing detoxification in sublethal endpoint and promotion in sublethal and lethal endpoints.(4) Using battery of small scale bioassays including Microtox, algal growth inhibition test, umu test, hydra acute test and daphinia acute test, the effects of 20 industrial wastewater were assessed. 85% of the samples showed any adverse effect. The causal substance for toxicity was evaluated by using the addition of chlating agent and comparing the analytical values.

(1)探讨了减少挥发性样品对微板测藻的影响的方法。使用天花板胶带和聚四氟乙烯片防止相邻威尔斯孔的污染，发现这不仅减少了挥发性样品的影响，而且与常规方法相比降低了细胞产率。这些操作对IC 50（50%抑制浓度）没有显著影响，表明这些技术将改善微孔板法的性能。(2)为了提高umu检测的效率，建立了96孔威尔斯微孔板法。对原方法的主要修改包括试剂和细菌培养物的体积、孵育温度、SDS浓度、吸光度测定波长等。（3）探讨了基于形态学变化的水螅急性毒性试验的敏感性。96 h-EC_（50）值对Cr ~（6+）具有较高的敏感性。对于某些金属，这种作用是短暂的，在亚致死终点表现出解毒作用，在亚致死和致死终点表现出促进作用。(4)采用Microtox、藻类生长抑制试验、umu试验、水螅急性试验和水蚤急性试验等一系列小规模生物测定方法，对20种工业废水进行了评价。85%的样本显示出任何不良反应。通过添加螯合剂和比较分析值来评价毒性的致病物质。