Study on neural subtrates involved in taste-aversion learning by means of molecular biology.

利用分子生物学研究参与味觉厌恶学习的神经底物。

• 批准号: 06680789
• 负责人: OGAWA Hisashi
• 金额: $ 1.41万
• 依托单位: KUMAMOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06680789/
• 关键词: PARABRACHIAL NUCLEUS; CEREBRAL CORTEX; MOUSE; FOS-PROTEIN; C-FOS; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; insituhybridization; 味覚野; 痙攣; Fos蛋白質; in-situ hybridization

Formation of learning involves increase in production of protein, mRNAs and so forth in response to stimuli. Yamamoto et al. (1993) reported about FOS-protein in the parabrachial nucleus (PBN) of rats in reponse to various taste solution in normal rats, and to conditoned taste after the animal learned conditioned taste-aversion (CTA). In the present project, we studied the production of FOS protein in insular cortex including the gustatory cortical area in mice at CTA formation, in reference to that in the PBN.When mice drunk basic taste solution, different taste induced FOS protein in different subnucleus of the PBN : NaCl, sucrose snd HCl induced many FOS-positive neurons at the lateral central subnucleus, and HCl produced them at the ventral subnucleus, as well. Quinine yielded a few FOS-postive neurons at the lateral external subnucleus. No FOS-positive neuron was found at the region where taste relay neurons have been located previously. In rats, FOS-positive neurons were induced … More in some other subnucleus, as well. In the insular cortex, sucrose induced many FOS-positive neurons at primary somatosensory area and granular insular cortex, while other taste stimuli including NaCl a fewer number of such neurons in the dysgranular insular cortex. After mice received salty taste and visceral illness by drinking LiCl, FOS-posiitive cells were seen at the lateral central and lateral external subnucleus of the PBN.Intraperitoneral injection of LiCl which induces only visceral illness produced FOS-positive neurons only at the lateral external subnucleus. In both cases, FOS-positive cells were noted at the dysgranular insular cortex. After mice learned CTA to NaCl or sucrose by associating it with visceral illness, either conditioned stimulus induced FOS-positive cells at the lateral central and ventral subnucleus of the PBN,and at the dysgranular cortex, though such a stimulus induced them only at the lateral external nucleus of the PBN.These findings were confirmed by observing induction of c-fos mRNA by in situ hybridization.The present results indicate that neural circuits involved in CTA differ in some degree between mice and rats. Less

学习的形成涉及对刺激做出反应的蛋白质、mRNA等的产生的增加。Yamamoto等（1993）报道了大鼠臂旁核（PBN）中的FOS蛋白在正常大鼠对各种味觉溶液的反应中以及在动物学习条件性味觉厌恶（CTA）后对条件性味觉的反应中的表达。本课题以小鼠外周核为参照，研究了CTA形成时，小鼠岛叶皮质包括味觉皮质区FOS蛋白的产生。当小鼠饮用基础味觉溶液时，不同味觉在外周核不同亚核中诱导FOS蛋白的产生：NaCl、蔗糖和HCl均能在中央外侧亚核产生大量的FOS阳性神经元，HCl也能在腹侧亚核产生FOS阳性神经元。奎宁在外侧外亚核产生了一些FOS阳性神经元。在味觉中继神经元所在的区域未发现FOS阳性神经元。在大鼠中，诱导FOS阳性神经元 ...更多信息 在其他亚核中也是如此在岛叶皮层中，蔗糖诱导初级躯体感觉区和颗粒岛叶皮层出现较多的FOS阳性神经元，而其他味觉刺激（包括NaCl）则诱导颗粒异常岛叶皮层出现较少的FOS阳性神经元。实验结果表明，在实验组小鼠的PBN中央外侧亚核和外侧外亚核内，均有FOS阳性神经元出现，而在实验组小鼠的PBN中央外侧亚核和外侧外亚核内，仅在外侧外亚核内出现FOS阳性神经元。在这两种情况下，注意到在颗粒异常岛叶皮质的福斯阳性细胞。在小鼠通过将其与内脏疾病相关联来学习CTA到NaCl或蔗糖之后，条件刺激在PBN的外侧中央和腹侧亚核以及在颗粒异常皮层诱导了FOS阳性细胞，虽然这种刺激只在PBN的外侧外核诱导它们，这些发现通过观察c-本研究结果表明，参与CTA的神经回路在小鼠和大鼠之间存在一定程度的差异。少

项目成果

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Ogawa,H.,Hasegawa,K.,Otawa,S.,Nakamura,T.: "Olfaction & Taste Xl,(Eds)Kurihara,K.,Suzuki,N.,Ogawa,H." Springer-Verlag, 864 (1994)

小川，H.，长谷川，K.，渥太华，S.，中村，T.：“嗅觉

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