Mechanism of neural regulation of the sciatic nerve blood flow

坐骨神经血流的神经调节机制

• 批准号: 06680820
• 负责人: SATO Yuko
• 金额: $ 1.47万
• 依托单位: Tsukuba College of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06680820/
• 关键词: Nerve blood flow; Sciatic nerve; Laser Doppler flowmetry; Axon reflex; Acetylcholine; CGRP; Rat; 血管収縮; 血管拡張

1. The response of nerve blood flow (NBF) in the sciatic nerve to repetitive electrical stimulation of either the ventral or dorsal root of the spinal nerves between the llth thoracic and 2nd sacral spinal segments, was examined using laser Doppler flowmetry. (1) Stimulation of the T11-L1 ventral roots produced a biphasic NBF response comprised of an initial increase and a subsequent decrease. The initial increase was a passive vasodilation due to the increase in mean arterial pressure (MAP), while the following decrease in NBF resulted from active vasoconstriction of the vasa nervorum due to the activation of sympathetic nerves innervating the sciatic vasa nervorum. (2) Stimulation of the ventral root of the L6 segment produced an increase in NBF,even though MAP decreased. This increase in NBF was abolished by i. v. injection of atropine, a muscarinic cholinergic receptor antagonist. (3) Stimulation of the dorsal roots between the L3 and S1 segments produced an increase in NBF,indepen … More dent of changes in MAP.This increase in NBF was abolished by topical application of a calcitonin generelated peptide (CGRP) receptor antagonist. In conclusion, NBF in the sciatic nerve is regulated by (1) sympathetic vasoconstrictors exiting the ventral roots of the spinal cord via the T11-L1 segments, (2) parasympathetic vasodilators exiting the ventral roots of the spinal cord via the L6 segment and (3) afferent CGRP containing vasodilators entering the dorsal roots of the spinal cord via the L3-S1 segments.2. The physiological relevance of peptidergic afferent vasodilative fibers in the regulation of blood flow in the vasa nervorum, with special reference to the axon reflex, was examined. The response of NBF in the sciatic nerve to electrical stimulation of saphenous nerve afferents was examined in anesthetized rats whose lumbosacral afferents and efferents had been disconnected from the spinal cord. Repetitive electrical stimulation of unmyelinated fibers in the central cut end of the saphenous nerve produced an increase in NBF in the sciatic nerve ipsilateral to the stimulation, independent of changes in MAP.This increase was abolished by topical application of CGRP receptor antagonest. In conclusion, NBF in the sciatic nerve is regulated via an axon reflex-like mechanism by unmyelinated afferent CGRP containing vasodilators with collaterals in the saphenous nerve. Less

1.用激光多普勒血流仪检测了重复电刺激第11胸段和第2骶段脊神经前根或后根时坐骨神经内神经血流的反应。(1)刺激T11-L1腹根产生一个双相NBF反应，包括初始增加和随后减少。最初的增加是由于平均动脉压（MAP）的增加而引起的被动血管舒张，而随后的NBF减少是由于支配坐骨神经血管的交感神经的激活而引起的神经血管的主动血管收缩。(2)刺激L 6节段的前根产生了NBF的增加，即使MAP下降。这一增长在NBF被取消i。静脉注射阿托品，一种毒蕈碱胆碱能受体拮抗剂。(3)刺激L3和S1节段之间的背根可使NBF增加，这与刺激L3节段和S1节段之间的背根有关。 ...更多信息 局部应用降钙素基因相关肽（CGRP）受体拮抗剂可消除NBF的增加。结论：坐骨神经NBF的调节机制为：（1）交感神经缩血管剂通过T11-L1节段流出脊髓前根，（2）副交感神经血管扩张剂通过L 6节段流出脊髓前根，（3）含有CGRP的血管扩张剂通过L3-S1节段进入脊髓后根.肽能传入血管舒张纤维的生理相关性在血管神经的血流调节，特别是轴突反射，进行了检查。在腰骶部传入和传出神经与脊髓切断的麻醉大鼠，观察了坐骨神经NBF对电刺激隐神经传入的反应。重复电刺激隐神经中央切断端的无髓纤维可使刺激同侧坐骨神经的NBF增加，而与MAP的变化无关，这种增加可被局部应用CGRP受体拮抗剂所消除。总之，坐骨神经中的NBF通过轴突反射样机制由隐神经中含有侧支血管扩张剂的无髓传入CGRP调节。少

项目成果

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