Eating behavior and fat transport from the interstine in old and obese rats

老年和肥胖大鼠的饮食行为和肠内脂肪转运

• 批准号: 12670074
• 负责人: SATO Yuko
• 金额: $ 2.18万
• 依托单位: Tokyo Metropolitan Institute of Gerontology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670074/
• 关键词: triglyceride; CCK-A receptor; Apolipoprotein-A; NPY; Intestinal transport; diacylglycerol; energy expenditure; apolipoproteinA-IV

OLETF rats are a model for NIDDM, which is first manifested at 18 weeks of age. We assessed age-related changes in lymphatic lipid transport in the intestine of OLETF rats and compared them with those of control LETO rats. Olive oil was infused into thterats with a mesenteric lymph fistula, which was created under ethrane anesthesia. A significant increase in lymphatic triglyceride transport in OLETF rats was observed at 18-19 weeks of age, compared with under 17 weeks of age, but no age-related change was observed in LETO rats. Food restriction, exercise training, or troglitazone treatment in OLETF rats prevented the age related increase in lipid transport. Biliary phosphatidylcholine concentration was higher in OLETF rats than in LETO rats, but no difference was seen in bile acid concentrations of the activity of microsomal TG transfer protein between the two strains. This study shows that increased lipid transport in the intestine may occur in NIDDM.The diurnal profile of energy expenditure in OLETF rats was different from LETO rats after NIDDM manifested. Treatments with food restriction, exercise, or troglitazone normalized diurnal profiles.Oral administration of diacylglycerold increased CCK release in humans, but the response was not different from that induced by triglyceride.Gallstone formation was observed when they were raised with high fat and high protein diet in CCK-A receptor knockout mice.

OLETF 大鼠是 NIDDM 的模型，其首次出现于 18 周龄。我们评估了 OLETF 大鼠肠道中淋巴脂质转运与年龄相关的变化，并将其与对照 LETO 大鼠进行比较。将橄榄油注入具有肠系膜淋巴瘘的患者中，该瘘是在乙烷麻醉下创建的。与 17 周龄以下相比，在 18-19 周龄时观察到 OLETF 大鼠的淋巴甘油三酯转运显着增加，但在 LETO 大鼠中未观察到与年龄相关的变化。 OLETF 大鼠的食物限制、运动训练或曲格列酮治疗可防止年龄相关的脂质转运增加。 OLETF大鼠的胆汁磷脂酰胆碱浓度高于LETO大鼠，但两种品系之间微粒体TG转移蛋白活性的胆汁酸浓度没有差异。本研究表明，NIDDM 可能发生肠道脂质转运增加。NIDDM 出现后，OLETF 大鼠的每日能量消耗情况与 LETO 大鼠不同。通过限制食物、运动或曲格列酮治疗使昼夜规律正常化。口服二酰基甘油会增加人类的 CCK 释放，但其反应与甘油三酯诱导的反应没有不同。在 CCK-A 受体敲除小鼠中，用高脂肪和高蛋白饮食喂养时，观察到胆结石形成。

项目成果

宮坂京子, 太田稔, 船越顕博: "PPARγアゴニスト、ピオグリタゾンの脂肪吸収、エネルギー代謝におよぼす影響"消化と吸収. 24. 44-48 (2001)

Kyoko Miyasaka、Minoru Ota、Akihiro Funakoshi：“PPARγ 激动剂吡格列酮对脂肪吸收和能量代谢的影响”《消化与吸收》24. 44-48 (2001)。

Miyasaka K, et al.: "Central orexin-A stimulates pancreatic exocrine secretion via vagus."Pancreas. (in press). (2002)

Miyasaka K 等人：“中枢食欲素-A 通过迷走神经刺激胰腺外分泌分泌。”胰腺。

Miyasaka K, et al.: "Amylase secretion from dispersed human pancreatic acini : Neither CCK-A nor CCK-B receptors mediate amylase secretion in vitro"Pancreas. (in press). (2002)

Miyasaka K 等人：“分散的人胰腺腺泡的淀粉酶分泌：CCK-A 和 CCK-B 受体都不介导体外淀粉酶分泌”胰腺。

Suzuki S, Miyasaka K et al.: "Importance of CCK-A receptor for gallbladder contraction and pancreatic secretion : A study in CCK-A receptor knockout mice"Jpn J Physiol. 51. 585-590 (2001)

Suzuki S、Miyasaka K 等：“CCK-A 受体对胆囊收缩和胰腺分泌的重要性：CCK-A 受体敲除小鼠的研究”Jpn J Physiol。

Miyasaka K, et al.: "Increased energy metabolism and energy turnover in mice lacking cholecystokinin-B receptor."J. Nutr.. (in press). (2002)

Miyasaka K 等人：“缺乏胆囊收缩素 B 受体的小鼠能量代谢和能量周转增加。”J.