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Genetic analysis of self-compatibility in a near-inbred of a wild potato species and construction of a genetic map

野生马铃薯近交系自交亲和性的遗传分析及遗传图谱构建

基本信息

批准号：
06806001
负责人：
HOSAKA Kazuyoshi
金额：
$ 1.34万
依托单位：
Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06806001/
关键词：
Potato Linkage map RFLP RAPD Self-incompatibility

项目摘要

A self-compatible (SC) variant of a wild diploid potato species Solanum chacoense, which is normally self-incompatible (SI), was investigated for self-compatibility. It was crossed with a SI cultivated diploid potato species S.phureja. The F_1 progenies segregated to be SC vs.SI and diallele crosses were made among them. Self-compatibility was tested in a selfed family of a parental SC variant and in sib-mated and selfed families of F_1 progenies. All the data suggest that there is a single dominant gene (Sli gene) inhibiting S gene expression in the pollens. Plants having a Sli gene produced pollens which are compatible to both its own parent and the others. The Sli gene has been maintained as a heterozygous condition through 8 times of selfed generations implying that homozygotes might be associated with lethality.One of the F_1 progenies was crossed as a male parent with one of selective clones from a S.stenotomum-S.phureja population, resulting in a segregating population consisted with 116 hybrid plants. Self-compatibility was assessed by selfing each of the hybrids. Sixty-six of them were SC,while 35 were SI,showing a significant distortion from an expected Mendelian ratio of 1 : 1. A genetic linkage map was constructed using DNA markers to localize the Sli gene. A total of 38 RAPD and 35 RFLP markers identified 52 loci on 11 linkage groups, among which the Sli gene was mapped at a distal end of chromosome 12. As A locus has been localized on chromosome 1 on the potato RFLP map, it is confirmed that the Sli gene is independent to S locus.

对野生二倍体马铃薯品种 Solanum chacoense 的自交亲和 (SC) 变体进行了研究，该变体通常是自交不亲和 (SI) 的。它与 SI 栽培的二倍体马铃薯品种 S.phureja 杂交。 F_1后代被分离为SC与SI，并且在它们之间进行双等位基因杂交。在亲本 SC 变体的自交家庭以及 F_1 后代的同胞交配和自交家庭中测试了自交亲和性。所有数据表明，花粉中存在一个抑制S基因表达的显性基因（Sli基因）。具有Sli基因的植物产生与其亲本和其他亲本均相容的花粉。 Sli基因在8次自交世代中一直保持杂合状态，这意味着纯合子可能与致死率相关。其中一个F_1后代作为父本与来自S.stenotomum-S.phureja群体的选择性克隆之一杂交，产生由116株杂种植物组成的分离群体。通过每个杂种自交来评估自交亲和性。其中 66 个为 SC，35 个为 SI，与预期的孟德尔比例 1:1 存在显着偏差。利用 DNA 标记构建遗传连锁图谱来定位 Sli 基因。总共38个RAPD和35个RFLP标记鉴定出11个连锁群上的52个位点，其中Sli基因定位于12号染色体远端。由于A位点在马铃薯RFLP图谱上定位于1号染色体上，因此证实Sli基因与S位点无关。