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Origin of T-type chloroplast DNA in potato

马铃薯T型叶绿体DNA的起源

基本信息

批准号：
12660006
负责人：
HOSAKA Kazuyoshi
金额：
$ 1.02万
依托单位：
Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

Chioroplast DNA of the common potato is different from that of its immediate ancestor, the Andean tetrapiold cultivated potato, by the presence of a 241 bp deletion and named T-type chioroplast DNA. To explore the ancestral wild species that conferred T-type chioroplast DNA to the cultivated potatoes, 566 accessions of 35 wild species from Bolivia and Argentina were surveyed for the presence/absence of the deletion by a simple PCR-based assay. The deletion was found in 16 accessions of S, berthaulti, S.neorossli and S.tarijense. The sequencing analysis of the deleted region confirmed that the 241 bp deletion occurred in the same region. In addition to the presence/absence of the deletion, two PCR-RFLP markers (H2 and H3) and four microsatellite markers (NTCP6, NTCP7, NTCP1.4 and NTCP18) were used to evaluate S. berthaultii (30 accessions), S. neorossii (five accessions), S. tarijense (62 accessions), putative S. berthaultiix S. tarijense hybrids (25 accessions), S. tuberosum ssp. tubetosum (7 accessions mostly from Chile) and ssp. andigena (five accessions, all having T-type chioroplast DNA), and one accession from each of S. stenotomum, S. phureja and S. chacoense. Twenty-five haplotypes were distinguished. All of the S. tuberosum accessions having T-type chtoroplast DNA were distinguished as haplotype 1 holders. The haptotype 1 was also found in 14 accessions of S. tarijense and four accessions of S. berthaultiix S. tarijense hybids. This strongly suggests that the wild maternal ancestor of common potato was S. tarijense.

普通马铃薯的叶绿体DNA与其直系祖先安第斯四倍体栽培马铃薯不同，存在241 bp的缺失，并被命名为t型叶绿体DNA。为了探索将t型叶绿体DNA传递给栽培马铃薯的祖先野生物种，我们对玻利维亚和阿根廷35种野生物种的566份材料进行了简单的pcr检测，以确定是否存在缺失。在S、berthaulti、S.neorossli和S.tarijense的16份材料中发现了缺失。对缺失区域的测序分析证实，241 bp的缺失发生在同一区域。利用2个PCR-RFLP标记（H2和H3）和4个微卫星标记（NTCP6、NTCP7、NTCP1.4和NTCP18），分别对30个品种的柏thaultii、5个品种的neorossii、62个品种的tarijense、25个品种的berthaultiix、25个品种的S. tuberosum进行鉴定。管茄（7份，主要来自智利）和ssp。andigena(5份，均为t型叶绿体DNA)， S. stenotomum、S. phureja和S. chacoense各1份。共鉴定出25个单倍型。所有具有t型叶绿体DNA的结核葡萄材料都被区分为单倍型1持有者。在14份牛蒡子材料和4份牛蒡子杂交材料中也发现了1型。这有力地表明，普通马铃薯的野生母系祖先是马铃薯。