Identification of multi-components of insect sex pheromones on the basis of recognition of mass spectral pattern.

基于质谱模式识别的昆虫性信息素多组分鉴定

• 批准号: 06806015
• 负责人: HORIIKE Michio
• 金额: $ 0.9万
• 依托单位: Kochi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06806015/
• 关键词: Fuzzy classification; recognition of pattern; insect sex pheromone; tetradecenyl acetate; double bond position; mass chromatography; mass spectral pattern; ファジィ分類法; マイクロマトグラフィー; ファジ-分類; マススペクトル

Most female insect sex pheromones, especially of Lepidopterous species are unsaturated straight-chain compounds. The major problem in elucidation of the chemical structure of insect sex pheromones is that the direct and unequivocal location of the double bond is often not easy. The present strategy for characterization of double bond position depends on a fuzzy classificational interpretation, in which EI mass spectra of chemically unmodified alkenyl compounds are analyzed on the basis of recognition of mass spectral patterns of a whole mass spectrum. A new method for elucidation of double bond position in binary mixtures of chromatographically unresolved isomeric tetradecenyl acetates was developed by the combination of mass chromatography and the fuzzy classification. The variation in isomer with the highest similarity index at the various scan points in mass chromatograms of the binary mixtures provided a basis for identification of components.In conclusion, the combination of the fuzzy classification and mass chromatography is also useful for the rapid analysis of some binary mixtures of tetradecenyl acetates, i.e.DELTA^3 and DELTA^5, DELTA^4 and DELTA^6, DELTA^7 and DELTA^9, DELTA^9 and DELTA^<11>, DELTA^7 and DELTA^<11>.

大多数雌虫性信息素，特别是鳞翅目昆虫的性信息素是不饱和直链化合物。解释昆虫性信息素的化学结构的主要问题是双键的直接和明确的位置往往并不容易。目前表征双键位置的策略依赖于一种模糊分类解释，即在识别整个质谱图的基础上分析化学上未经修饰的烯基化合物的EI质谱图。提出了一种用质谱法和模糊聚类相结合的方法来确定未拆分的十四烯基乙酸酯二元混合物中的双键位置。结果表明，模糊分类与质谱分析相结合的方法同样适用于DELTA^3与Delta^5、Delta^4与Delta^6、Delta^7与Delta^9、Delta^9与Delta^11、Delta^7与Delta^11&gt；