Study on the chemicals controlling insect behavior in the Japanese cedar, Cryptomeria japonica

控制日本柳杉昆虫行为的化学物质研究

• 批准号: 12839012
• 负责人: HORIIKE Michio
• 金额: $ 2.37万
• 依托单位: Kochi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12839012/
• 关键词: antifeedant; Cryptomeria japonica; Acuta despesta; (-)-cubebol; (+)-2.7(14), 10-bisabolatrien-1-ol-4-one; sequirin-C; agathresinol; (-)-cubebol; (+)-2, 7, (14), 10-bisabola trien-1-01-4-one; Seguirin-C; agathresino; Acusta despesta; (+)-2,7(14

During our studies on the components in Japanese cedar, Cryptomeria japonica, we found that the crude methanol extract of C. japonica showed intense antifeeding activity against one snail species, Acusta despesta, which is well-known as a pest of many vegetables and crops. The active components in the extract were separated into the hexane and ethyl acetate soluble fractions. From the active ethyl acetate soluble fraction, two norlignans, sequirin-C and agatharesinol, were isolated and identified as the active compounds. Both compounds inhibited feeding behavior of A. despesta at 30 μg/cm2 and 40 μg/cm2 concentrations, respectively, when applied by an eggplant leaf or filter paper containing-20 μl of 5 % sucrose solution.On the other hand, (-)-cubebol and (+)-2,7(14),10-bisabolatrien-1-ol-4-one were isolated and identifed as the active compounds from the hexane fr. Both compounds strongly inhibited the feeding behavior of A. despesta at 120 μg/cm2 and 80 μg/cm2 concentrations, respectively. In-addition to these, the absolute configuration of (+)-2,7(14), 10-bisabolatrien-1-ol-4-one was also determined as (IS, 6R)-2,7(14),10-bisabolatrien-1-ol-4-one.

在对日本杉木（Cryptomeria japonica）成分的研究中，我们发现日本杉木的粗甲醇提取物对一种蜗牛（Acusta despesta）具有很强的抗取食活性，这种蜗牛是许多蔬菜和作物的害虫。提取液中有效成分分离为己烷和乙酸乙酯可溶性部分。从活性乙酸乙酯可溶性组分中分离得到两种降木脂素，分别为sequinin - c和agatharesinol。两种化合物分别在含有-20 μl 5%蔗糖溶液的茄子叶或滤纸上施用30 μg/cm2和40 μg/cm2时，抑制了白腹蚜的取食行为。从己烷中分离得到(-)-cubebol和(+)-2、7（14）、10-双abolatrien-1-ol-4-one，在浓度分别为120 μg/cm2和80 μg/cm2时，这两种化合物均具有较强的抑制食蚜行为的作用。此外，(+)-2,7(14)，10-双abolatrien-1-ol-4-one的绝对构型也确定为(IS, 6R)-2,7(14)，10-双abolatrien-1-ol-4-one。

项目成果

Xiao Hui Chen: "Antifeedants Against Acusta despesta from the Japanese cedar, Crypfomeria japonica"Zeitschrift fur Naturforschung. (in press). (2001)

陈晓辉：“针对来自日本雪松、Crypfomeria japonica 的 Acusta desspesta 的拒食剂”Zeitschrift Fur Naturforschung。

Xiao Hui Chen et al.: "Antifeedant Against Acusta despesta from the Japanese cedar, Cryptomeria Japonica"Zeitschrift fur Naturforschung. 56C. 249-252 (2001)

陈晓辉等人：“针对来自日本雪松、柳杉的 Acusta desspesta 的拒食剂”Zeitschrift Fur Naturforschung。

Xiao Hui Chen et al.: "Antifeedant Against Acusta despesta from the Japanese cedar, Cryptomeria Japonica"Bioscience, Biotechnology, and Biochemistry. 65(6). 1434-1437 (2001)

陈晓辉等人：“来自日本雪松、柳杉的拒食剂”生物科学、生物技术和生物化学。

Xiao Hui Chen, Chul-sa Kim, Takehiro Kashiwagi, Shin-ichi Tebayashi and Michio Horiike: "Antifeedants against Acusta despesta from the Japanese Cedar, Cryptomeria japonica"Zeitschrift fur Naturaforschung. 56e. 249-252 (2001)

陈晓辉、Chul-sa Kim、Takehiro Kashiwagi、Shin-ichi Tebayashi 和 Michio Horiike：“针对来自日本雪松、日本柳杉的 Acusta despesta 的拒食剂”Zeitschrift Fur Naturaforschung。

Xiao Hui Chen, Chul-sa Kim, Takehiro Kashiwagi, Shin-ichi Tebayashi and Michio Horiike: "Antifeedants against Acusta despesta from the Japanese Cedar, Cryptomeria japonica II"Bbiosic Bbiotechnol. Biochem.. 65(6). 1434-1437 (2001)

陈晓辉、Chul-sa Kim、Takehiro Kashiwagi、Shin-ichi Tebayashi 和 Michio Horiike：“针对来自日本雪松、柳杉 II 的 Acusta desspesta 的拒食剂”Bbiosic Bbiotechnol。