Immuno-biochemical studies on a novel DNA tyge of bacteria from a kawasaki ob's ease patient and the role of a novel protease

1.对一株来自川崎病患儿的链球菌变异株进行了基因鉴定，明确了可能的致病抗原，包括一种新型的蛋白水解酶。在刺激性条件下的PNA-DNA断裂试验证实，可能的致病微生物肯定不同于一系列口腔链球菌。对16S RNA基因进行了测序，证实了5个突变Aite可用于RFLP检测一系列可复制性株的诊断。用特异性单抗也可在川崎病患者血清中检测到毒力抗原。

1. The genetic indentification of a variant streptococcal strain irolated from a Kawasaki disease child has benn successfully performed and the possible zixrulent antigens including a novel type of protease have been-clarified.2. The PNA-DNA hy brkidization test in a strimgent condition confirmed that the possibly causatine organism certainly differed from a serils of oral streptococci.3. The 16s RNA gene was sequenced and 5 mutation aites were elucidated to be useful for RFLP detectine diagnosis of a series of reproducibly virnlent strains.4. The virulent antigens were regroduibly detected in the sera of Kawasaki poctients also using the specific monoclonal antibodies.