The influence of arachidonic acid on radicular cysts and its decrease in response to root canal treatment

前列腺素是根囊肿周围骨吸收的加速剂，为了阐明根管治疗引起的根囊肿减少与花生四烯酸代谢之间的关系，对临床诊断为根囊肿的患者在不同的发病阶段前列腺素的变化进行了研究。在保存牙齿，根尖囊肿切除后，固定时间后根管充填的标准大小的方法。与此相反，不保守的牙齿，在根囊肿切除术中被拔出。以切除的包囊为实验材料。健康牙龈标本从个人患者作为对照。切除后立即进行原代培养。花生四烯酸代谢产物通过薄层色谱法进行分离和定性分析，然后用[ ^3H]花生四烯酸标记，然后测定放射性。与对照组（牙龈）相比，从根管充填到囊肿切除时间短的患者和接受根管治疗的患者的花生四烯酸代谢产物水平较高，而病程长的患者血药浓度有降低的趋势。未行根管治疗的患者，其根尖囊肿壁花生四烯酸代谢产物的水平随临床症状和分期的不同而不同，提示适当的根管治疗有抑制根尖囊肿壁花生四烯酸代谢的趋势。这一趋势需要通过研究更多不同临床阶段的患者来证实，并且将来应该评估根尖病变牙髓计数器的有效性。

Changes in prostaglandin, an accelerator of bone absorption around radicular cysts, were studied in various morbid stages to elucidate the relationship between decreases in radicular cysts due to root canal treatment, a phenomenon which has been observed clinically, and arachidonic acid metabolism.The subjects were patients with clinically diagosed radicular cysts. In conservable teeth, the radicular cyst was excised after a fixed period following root canal filling by the standard size method. Unconservable teeth, in contrast, were extracted during excision of the radicular cyst. The ecised cysts were used as experimental materials. Healthy gingival specimens form individual patients served as controls. Immediately after the excision, primary culture wasperformed. Metabolites of arachidonic acid were subjected to isolation and qualitative analysis by thin-layer chromatography followed by labeling with[ ^3H]arachidonic acid, and then determination of radioactivity.The level of arachidonic acid metabolites was higher in patients in whom the period from root canal filling to cyst excision was short and in patients who underwent root canal treatment as compared to the controls(gingiva), while the level tended to be low in patients in whom the period was long. In other patients who did not undergo root canal treatment, the level of arachidonic acid metabolites varied with clinical symptom and stages.These results suggest that appropriate root canal treatment tends to suppress metabolism of arachidonic acid on the radicular cyst wall. This tendency needs to be confirmed by studying more patients in various clinical stages, and the validity of an endodontic counter for apical leasion should be evaluated in the future.