Mass Spectrometry Platform (Part II): Ion Mobility Q-TOF Mass Spectrometer with Nano-HPLC System

质谱平台（第二部分）：配备 Nano-HPLC 系统的离子淌度 Q-TOF 质谱仪

• 批准号: 445105248
• 金额: --
• 依托单位: Martin-Luther-Universität Halle-Wittenberg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/445105248?language=en
• 关键词: Mass; Spectrometry; Platform; Part; II

The mass spectrometry platform described herein will be used for protein structure analysis in the recently established “Center for Structural Mass Spectrometry” (CSMS) headed by Prof. Sinz. The equipment will serve to conduct cooperative research projects with the Faculty of Natural Sciences I and the Medical Faculty at the Martin Luther University Halle-Wittenberg as well as within the RTG 2467 “Intrinsically Disordered Proteins – Molecular Principles, Cellular Functions, and Diseases” to investigate three-dimensional protein structures and to delineate protein-protein interactions. Both LC/MS systems have to fulfill all requirements to guarantee a robust, high-throughput analysis of proteins. There will be a clear separation of projects for the two ion mobility Q-TOF mass spectrometers in the CSMS: One LC/MS system will be employed for cross-linking/MS experiments, while the second LC/MS system will be used for conducting H/D exchange experiments. This will allow obtaining complementary data for the three-dimensional structural analysis of proteins and protein complexes up to system-wide protein network analyses. Establishing the CSMS will further strengthen the research focus on protein mass spectrometry at the MLU Halle-Wittenberg.

本文描述的质谱平台将在最近成立的由Sinz教授领导的“结构质谱中心”（CSMS）中用于蛋白质结构分析。该设备将用于与哈雷-维滕贝格马丁路德大学自然科学学院和医学院以及 RTG 2467“本质无序蛋白质 - 分子原理、细胞功能和疾病”开展合作研究项目，以研究三维蛋白质结构并描绘蛋白质-蛋白质相互作用。两种 LC/MS 系统都必须满足所有要求，以保证对蛋白质进行稳定、高通量的分析。 CSMS 中的两台离子淌度 Q-TOF 质谱仪的项目将有明确的分离：一套 LC/MS 系统将用于交联/MS 实验，而第二套 LC/MS 系统将用于进行 H/D 交换实验。这将允许获得蛋白质和蛋白质复合物三维结构分析直至全系统蛋白质网络分析的补充数据。 CSMS 的建立将进一步加强 MLU Halle-Wittenberg 的蛋白质质谱研究重点。