A versatile mass spectrometry platform for quantitative proteomics underpinning biomedical research at The University of Sheffield.

点击翻译按钮获取中文摘要

Proteins and metabolites best represent the state of a cell or organism at a given moment in time. The total collection of these proteins and metabolites (proteomes and metabolomes) is, therefore, a very informative readout of biology or an individual's health. Analysing proteins and metabolites is challenging due to the many different types and diverse characteristics. However, an analytical approach called mass spectrometry is well suited to both the identification and, importantly, quantification of a vast range of these biological molecules. We propose installing a versatile mass spectrometry platform in our mass spectrometry research facility to provide state-of-the-art proteome and metabolome analysis for researchers across the university. This instrument will allow us to increase the number of samples we can analyse and compare, perform a more detailed analysis of samples that are challenging with existing technology and permit precise measurement of specific sets of proteins and metabolites in a range of samples.Researchers at The University of Sheffield will use this technology to understand the molecular processes underlying antimicrobial resistance, neurodegeneration and cancer and to identify molecular changes in patient samples to develop and test novel drugs to treat a range of diseases.