Cross-kingdom RNA transfer in the barley-powdery mildew interaction

大麦-白粉病相互作用中的跨界RNA转移

• 批准号: 447519304
• 负责人: Professor Dr. Ralph Panstruga
• 金额: --
• 依托单位: AG Panstruga (Molekulare Zellbiologie)
• 依托单位国家: 德国
• 项目类别: Research Units
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/447519304?language=en
• 关键词: Cross; kingdom; RNA; transfer; barley

Powdery mildew fungi are fungal (ascomycete) phytopathogens that can infect a broad range of angiosperm plants and cause substantial damage on crop and ornamental plants. They thrive on the basis of an obligate biotrophic lifestyle, i.e. they require living host plants for growth and reproduction. The interaction between barley (Hordeum vulgare) and its powdery mildew pathogen, Blumeria graminis f.sp. hordei (Bgh), is highly-coevolved, agronomically relevant and well-characterized at the phytopathological, genetic, molecular and cellular level. The recent discovery of small RNAs (sRNAs) with cross-kingdom RNA transfer potential in both partners of the interaction raises the intriguing possibility that extracellular sRNAs modulate the plant-powdery mildew warfare. Here, we propose a research program that aims at elucidating the supposed role of extracellular sRNAs in the barley-Bgh and Arabidopsis thaliana-Golovinomyces orontii interactions. In particular, we wish to identify sRNAs of the respective interaction partners and functionally characterize them in the context of infection. In addition, we want to clarify whether extracellular vesicles mediate the presumed mutual exchange of extracellular sRNAs and further analyze the putative role of the abundant Bgh RNase-like effector proteins (termed RALPHs) therein. Our studies will reveal whether and to which extent extracellular sRNAs play a role in the interaction of a crop plant with an obligate biotrophic pathogen and if host and microbial sRNA targets are conserved in comparison to other pathosystems studied within our consortium

白粉病真菌是真菌（子囊菌）植物病原体，可感染广泛的被子植物，并对农作物和观赏植物造成严重损害。它们在专性生物营养生活方式的基础上茁壮成长，即它们需要活的宿主植物进行生长和繁殖。大麦（Hordeum vulgare）与其白粉病病原菌大麦白粉病菌（Blumeria graminis f.sp. hordei，Bgh）之间的相互作用是高度共进化的，具有农艺学相关性，并在植物病理学、遗传学、分子和细胞水平上得到了充分的表征。最近发现的小RNA（sRNA）与跨王国的RNA转移潜力的相互作用的合作伙伴提出了有趣的可能性，胞外sRNA调节植物白粉病战争。在这里，我们提出了一个研究计划，旨在阐明假定的作用，细胞外sRNAs在大麦BGH和拟南芥Golovinomyces orontii相互作用。特别是，我们希望确定各自的相互作用伙伴的sRNAs和功能的特点，他们在感染的情况下。此外，我们想澄清是否细胞外囊泡介导的假定相互交换的细胞外sRNA和进一步分析推定的作用，丰富的BGH RNase样效应蛋白（称为RALPHs）在其中。我们的研究将揭示胞外sRNA是否以及在何种程度上在作物与专性生物营养型病原体的相互作用中发挥作用，以及与我们研究的其他病理系统相比，宿主和微生物sRNA靶标是否保守