Functional analysis of the Arabidopsis thaliana Mlo (AtMlo) gene family

拟南芥Mlo (AtMlo)基因家族的功能分析

• 批准号: 5446497
• 负责人: Professor Dr. Ralph Panstruga
• 金额: --
• 依托单位: AG Panstruga (Molekulare Zellbiologie)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2004
• 资助国家: 德国
• 起止时间: 2003-12-31 至 2008-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5446497?language=en
• 关键词: Functional; analysis; Arabidopsis; thaliana; Mlo

MLO proteins constitute a family of plant-specific, heptahelical, plasma membrane-localized integral membrane proteins. Barley MLO, the "founder" of the protein family, serves a role as modulator of pathogen defence and cell death and is assumed to regulate a SNARE protein-dependent and vesicle-associated defence pathway at the cell periphery. We tested T-DNA insertion mutants in Arabidopsis Mlo genes for altered infection phenotypes upon challenge with a range of phytopathogens. We found that the AtMlo2 mu tant is allelic to the previously described pmr2 mutant and that AtMlo2 mutant alleles phenocopy the broad-spectrum powdery-mildew resistant phenotype of barley mlo mutants. This finding suggests a conserved role for MLO proteins in monocot and dicot plants and indicates that the ability of powdery mildew fungi to manipulate the MLO pathway has been retained for at least 150 million years. We will exploit this novel discovery to unravel the molecular basis of MLO function in Arabidopsis by various approache s including analysis of mlo signaling requirements, Atmlo2 suppressor mutagenesis, and comparative transcript profiling.

MLO蛋白是一类植物特异性的七螺旋质膜定位的膜整合蛋白。大麦MLO，蛋白质家族的“创始人”，作为病原体防御和细胞死亡的调节剂发挥作用，并被认为调节细胞外周的SNARE蛋白依赖性和囊泡相关防御途径。我们测试了拟南芥Mlo基因中的T-DNA插入突变体在用一系列植物病原体挑战后改变的感染表型。我们发现AtMlo 2突变体与先前描述的pmr 2突变体是等位的，并且AtMlo 2突变体等位基因表型复制大麦mlo突变体的广谱白粉病抗性表型。这一发现表明MLO蛋白在单子叶植物和双子叶植物中的保守作用，并表明白粉病真菌操纵MLO途径的能力已经保留了至少1.5亿年。我们将利用这一新的发现，通过各种方法，包括mlo信号需求分析，Atmlo 2抑制突变和比较转录谱分析，解开拟南芥中MLO功能的分子基础。