STED Superresolution and Confocal Microscope

柏林医学大学本杰明·富兰克林校区(CBF)的用户联盟(14个团体)申请使用STED超分辨率显微镜，用于细胞生物学、胃肠病学、肾脏、免疫学和肿瘤学研究项目。在CBF有一个共焦显微镜，然而，没有用于超分辨率荧光显微镜的STED显微镜，这项技术目前正在发展成为此类研究的标准。未来的用户参与了DFG和BMBF的众多单独和联合研究项目，因此迫切需要一台STED显微镜。此次收购服务于这些第三方资助项目的技术支持和方法进步。该显微镜将用于：超分辨率(超出阿贝极限)和高灵敏度的亚细胞结构成像、纳米结构蛋白质分布、多蛋白质复合体的组成。所研究的结构包括紧密连接、极性、受体和信号复合体、脂滴、线粒体、病毒、纳米颗粒。为此，将进行固定样品的2D/3D成像和动态细胞过程的活细胞成像。与目前可用的共焦显微镜相比，目前应用的STED显微镜将在低水平的光毒性/光漂白下实现相当高的空间分辨率。因此，它将允许高分辨率的共同本地化研究，这对我们的创新和高级别结构和功能研究项目的成功处理具有决定性意义。

The user consortium (14 groups) at the Campus Benjamin Franklin (CBF) of the Charité - Universitätsmedizin Berlin applies for a STED superresolution microscope for cell biological, gastroenterological, renal, immunological and oncological research projects. At the CBF there is one confocal micoscope, however, there is no STED microscope for superresolution fluorescence microscopy, a technique which is currently developing into a standard for such investigations. The future users are involved in numerous DFG and BMBF single and joint research projects, for which a STED microscope is urgently needed. The acquisition serves the technical support and methodological advancement of these third party funded projects. The microscope will be used for: superresolution (beyond the Abbe limit) and high-sensitivity imaging of subcellular structures, nanostructural protein distribution, composition of multi protein complexes. Structures investigated comprise tight junctions, polarity, receptor and signalling complexes, lipid droplets, mitochondria, viruses, nanoparticles. To this end 2D/3D-imaging of fixed samples and live cell imaging of dynamic cellular processes will be carried out. Compared to the currently available confocal microscope, the STED microscope presently applied for will enable considerably higher spatial resolution (< at low levels of photo-toxicity / photo-bleaching. Thus, it will allow high-resolution co-localization studies, which are decisive for the successful processing of our innovative and high-ranking structural and functional research projects.