Superresolution SIM/SMLM Fluorescence Microscope

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Super-resolution fluorescence microscopy (SRFM) has revolutionized the understanding in biology in many places, as it can be used to image dynamic processes in native environments with near molecular, but at least nanoscopic resolution. These resolution capabilities were previously reserved for electron microscopy (EM) or atomic force microscopy (AFM). These developments are massively supported by the development of commercial SRFMs, which feature increasingly sophisticated software and user-friendliness. In the areas of soft matter research, supramolecular systems, and polymer research, EM and AFM have dominated structural analysis to date, but most techniques can only study dried, surface-immobilized, or fixed samples (with slight exceptions for cryo-TEM or liquid-cell AFM). Confocal microscopy is used in parallel, but its resolving power follows the diffraction limit. However, imaging of dynamic processes with finest nanoscopic resolution and simultaneous imaging of hydrated states under dynamic changes is also of increasing importance in soft matter. There has also been seminal work on SRFM in soft matter research in recent years, particularly in the areas of supramolecular systems, polymer self-assembly, and DNA nanoscience, which clearly demonstrate that a completely new and deeper level of understanding can be achieved using SRFM methods. Therefore, the goal of this GG proposal is to purchase a SRFM instrument with combined Structured Illumination (SIM) and Single Molecule Localization Microscopy (SMLM) methodologies for the investigation of soft matter systems. This application is led by Prof. A. Walther, but the instrument will be affiliated with the Electron Microscopy Center Mainz and will be available to users in general. In this context, the AG Walther will also still fill an academic position (permanent position available), which will ensure the daily scientific and administrative operation. According to the research concepts, the main application fields are self-assembling and nanostructured systems from the fields of DNA nanoscience, polymer research, colloid chemistry, peptide nanostructures and biochemistry. The instrument will be strengthened with peripherals for measuring temperature-induced transitions and automated fluid routing for investigation of soft matter systems. Funding on the state side will come from a Gutenberg Research Professorship for Prof. Walther as part of his appointment to JGU Mainz.