Superresolution SIM/SMLM Fluorescence Microscope

超分辨率 SIM/SMLM 荧光显微镜

• 批准号: 497845157
• 金额: --
• 依托单位: Johannes Gutenberg-Universität Mainz
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2022
• 资助国家: 德国
• 起止时间: 2021-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/497845157?language=en
• 关键词: Superresolution; SIM; SMLM; Fluorescence; Microscope

Super-resolution fluorescence microscopy (SRFM) has revolutionized the understanding in biology in many places, as it can be used to image dynamic processes in native environments with near molecular, but at least nanoscopic resolution. These resolution capabilities were previously reserved for electron microscopy (EM) or atomic force microscopy (AFM). These developments are massively supported by the development of commercial SRFMs, which feature increasingly sophisticated software and user-friendliness. In the areas of soft matter research, supramolecular systems, and polymer research, EM and AFM have dominated structural analysis to date, but most techniques can only study dried, surface-immobilized, or fixed samples (with slight exceptions for cryo-TEM or liquid-cell AFM). Confocal microscopy is used in parallel, but its resolving power follows the diffraction limit. However, imaging of dynamic processes with finest nanoscopic resolution and simultaneous imaging of hydrated states under dynamic changes is also of increasing importance in soft matter. There has also been seminal work on SRFM in soft matter research in recent years, particularly in the areas of supramolecular systems, polymer self-assembly, and DNA nanoscience, which clearly demonstrate that a completely new and deeper level of understanding can be achieved using SRFM methods. Therefore, the goal of this GG proposal is to purchase a SRFM instrument with combined Structured Illumination (SIM) and Single Molecule Localization Microscopy (SMLM) methodologies for the investigation of soft matter systems. This application is led by Prof. A. Walther, but the instrument will be affiliated with the Electron Microscopy Center Mainz and will be available to users in general. In this context, the AG Walther will also still fill an academic position (permanent position available), which will ensure the daily scientific and administrative operation. According to the research concepts, the main application fields are self-assembling and nanostructured systems from the fields of DNA nanoscience, polymer research, colloid chemistry, peptide nanostructures and biochemistry. The instrument will be strengthened with peripherals for measuring temperature-induced transitions and automated fluid routing for investigation of soft matter systems. Funding on the state side will come from a Gutenberg Research Professorship for Prof. Walther as part of his appointment to JGU Mainz.

超分辨率荧光显微镜（SRFM）在许多地方已经彻底改变了对生物学的理解，因为它可以用于以接近分子但至少是纳米级的分辨率对天然环境中的动态过程进行成像。这些分辨率能力以前保留给电子显微镜（EM）或原子力显微镜（AFM）。这些发展在很大程度上得到了商业标准化资源管理系统的发展的支持，这些系统的特点是越来越复杂的软件和用户友好性。在软物质研究，超分子系统和聚合物研究领域，EM和AFM主导了结构分析，但大多数技术只能研究干燥，表面固定或固定的样品（低温TEM或液体细胞AFM略有例外）。共焦显微镜是并行使用的，但其分辨率遵循衍射极限。然而，在软物质中，具有最精细的纳米级分辨率的动态过程的成像和动态变化下的水合状态的同时成像也越来越重要。近年来，SRFM在软物质研究中也有开创性的工作，特别是在超分子系统，聚合物自组装和DNA纳米科学领域，这清楚地表明，使用SRFM方法可以实现全新和更深层次的理解。因此，本GG提案的目标是购买一台结合结构照明（SIM）和单分子定位显微镜（SMLM）方法的SRFM仪器，用于软物质系统的研究。该应用程序由A教授领导。Walther，但该仪器将隶属于美因茨电子显微镜中心，并将提供给一般用户。在这种情况下，AG瓦尔特还将填补学术职位（永久职位），这将确保日常的科学和行政运作。根据研究概念，主要应用领域是DNA纳米科学，聚合物研究，胶体化学，肽纳米结构和生物化学领域的自组装和纳米结构系统。该仪器将加强与外围设备，用于测量温度引起的转变和自动流体路由软物质系统的调查。国家方面的资金将来自瓦尔特教授的古滕贝格研究教授职位，这是他被任命为JGU美因茨大学的一部分。