Development of a high-resolution scanning electrochemical microscope to characterize micro-bio devices

开发高分辨率扫描电化学显微镜来表征微生物设备

• 批准号: 15201030
• 负责人: MATSUE Tomokazu
• 金额: $ 32.45万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15201030/
• 关键词: Scanning Probe Microscopy; Micro-nano device; Biophysics; Research Instrument for Biology; Cellular array chip; ultramicroelectrode; near-field microscopy; 走査型電気化学顕微鏡; ナノプローブ; ディップ・ペン・ナノリソグラフィー; GFP; DNA; 距離制御; バイオチツプ; 走査型近接場光学顕微鏡; 大腸菌; バイ

We developed a high-resolution scanning electrochemical microscope (SECM) towards characterization of various micro-bio devices. Our SECM-based multifunctional measuring system provides near-field scanning optical microscopic (NSOM) information by featuring an optical fiber-electrode probe. The size of the electrode-, optical fiber-, or optical fiber-electrode- probe is less than 100nm, which allows us high-resolution images of protein arrays and cellular arrays. The feedback mechanism controlling the probe-sample distance is based on the shearing force, by which high-quality images of rough surfaces with the height differences larger than 10μm and fragile samples such as living cells under physiological conditions were captured.1, Nano-electrode probes were fabricated with the Ti/P sputtering and the subsequent parylene C-vapor deposition polymerization. The effective electrode radius estimated from the cyclic voltammogram for ferrocyanide has found to be less than 50nm. The optical f … More iber-electrode probes with the radii less than 100nm were made as the same manner. The optical aperture size was also less than 100 nm which was confirmed from the cross section of the NSOM image of the quantum-dot (QD) particles with tens nm-diameters.2, The feedback mechanism controlling the probe-sample distance was improved by vertically moving the probe with the width of 〜 100nm to reduce the damage applied to the samples. This feedback mode, defined as "standing approach (STA) mode" (Yamada et al., Anal. Chem., 2005, 77, 1785-179), has allowed to simultaneous electrochemical and topographic images of the axon and the cell body of the single PC-12 cell under physiological conditions for the first time. The STA-mode feedback imaging sometimes works better than the tip-sample regulation for the commercially available AFM. For example, relatively larger polystyrene beads (with 〜 10μm diameter) can be imaged with our STA-mode SECM, whereas not imaged with the conventional AFM instrument.3, Towards the progress in the construction of the micro-bio devices, various techniques to pattern baiomaterials were attempted. Especially, collagen-gel embedded culture of bacteria and mammalian cells with ultra-small volume (tens to several nL) has been successfully demonstrated on glass or silicon chip to microfabricate various types of cellular arrays. Micro-contact printing (μCP), microfluidics, dip-pen nanolithography (DPN), and negative dielectric phoresis (n-DEP) have been also applied to assemble protein and cellular arrays. Less

我们开发了高分辨率扫描电化学显微镜（SECM），以表征各种微型生物设备。我们基于SECM的多功能测量系统通过具有光纤电极探针来提供近场扫描光学显微镜（NSOM）信息。电极，光纤或光纤 - 电极 - 探针的尺寸小于100nm，这使我们可以对蛋白质阵列和细胞阵列进行高分辨率图像。控制探针样本距离的反馈机制基于剪切力，该剪切力的高度表面的高质量图像大于10μm，并捕获了脆弱的样品，例如生理条件下的活细胞。1，Nano-Electrode问题与Ti/P Spters Sptersing和随后的Parylene C-Prarylene C-Promignization一起制造。从循环伏安图中估算的有效电极半径已发现小于50nm。光学f…较小的iber电极问题小于100 nm，以相同的方式使。光孔径的大小也小于100nm，这是从具有TENS NM直径的量子点（QD）颗粒的NSOM图像的横截面证实的。这种反馈模式定义为“站立进场模式”（Yamada等，Anal。Chem。，2005，77，1785-179），已允许在第一次在生理条件下首次在生理条件下同时进行轴突和单个PC-12细胞的细胞体的电化学和地形图。 STA模式反馈成像有时会比市售AFM的Tip-sample调节效果更好。例如，可以使用我们的STA模式SECM对相对较大的聚苯乙烯珠（直径约为10μm）进行成像，而不是使用常规AFM仪器成像。3，朝着Micro-Bio设备的构建过程中，尝试了各种技术进行贝伊材料的模式。尤其是，在玻璃或硅芯片上成功证明了具有超小体积的细菌和哺乳动物细胞的胶原凝胶嵌入的培养物，从而成功证明了各种类型的细胞阵列。微接触印刷（μCP），微流体，浸入式纳米函数（DPN）和阴性饮食光（N-DEP）也已用于组装蛋白质和细胞阵列。较少的

项目成果

Dielectrophoretic micropatterning with microparticle monolayers covalently linked to glass surfaces

• DOI: 10.1021/la048111p
• 发表时间: 2004-12-07
• 期刊: LANGMUIR
• 影响因子: 3.9
• 作者: Suzuki, M;Yasukawa, T;Matsue, T
• 通讯作者: Matsue, T

細胞の形質転換方法及び形質導入方法

细胞转化和转导方法

• 发表时间: 2005

Y.Torisawa: "Proliferation assay on a silicon chip applicable for tumors extirpated from mammalians"International Journal of Cancer. 109. 302-308 (2004)

Y.Torisawa：“适用于哺乳动物摘除肿瘤的硅芯片上的增殖测定”国际癌症杂志。

Fabrication of Microbial Chip Using Collagen Gel Microstructure.

使用胶原凝胶微结构制造微生物芯片。

• 发表时间: 2003
• 期刊: Lab on a Chip 3
• 作者: T.Kaya

Real-time monitoring of reactive oxygen species production during differentiation of human monocytic cell lines (THP-1)

• DOI: 10.1016/j.aca.2005.06.034
• 发表时间: 2005-09-06
• 期刊: ANALYTICA CHIMICA ACTA
• 影响因子: 6.2
• 作者: Kasai, S;Shiku, H;Yoshimura, T
• 通讯作者: Yoshimura, T