Novel electrode array device for electrochemical imaging

在这项研究中，我们提出了一种基于局部氧化还原循环的电化学（LRC-EC）装置和系统的电化学成像新方法。在该装置中，将两个微电极阵列正交排列，形成一个n × n的交叉点（测量点）阵列，只有2n个键垫用于外部连接。通过在列电极和行电极处设置适当的电位，在期望的交叉点处诱导局部氧化还原循环，可以很容易地解决柱电极和行电极的交叉点。因此，交叉点可以用作单独的电化学传感器。该可寻址的多重检测系统成功应用于酶活性检测、基因表达分析、细胞分化分析、DNA检测和液滴动态分析。LRC-EC系统将广泛应用于各种生物成像和生物传感。

In this study, we have proposed a novel method for electrochemical imaging using local redox cycling-based electrochemical (LRC-EC) devices and systems. In the device, two arrays of microelectrodes are orthogonally arranged to fabricate an n × n array of crossing points (measurement points) with only 2n bonding pads for external connection. The crossing points of the column and row electrodes can easily be addressed by setting the proper potentials at the column and row electrodes to induce local redox cycling at desired crossing points. Therefore, the crossing points can be used as individual electrochemical sensors. This addressable and multiple detection system successfully applied for the detection of enzyme activity, gene-expression analysis, cell differentiation analysis, DNA detection and dynamic analyses of droplets. The present LRC-EC system will be widely applicable for various bioimaging and biosensing.