Developments of Anti-tick Vaccine and Bio-acaricides Using Recombinant Virus of Tick Bioactive Molecules

利用蜱生物活性分子重组病毒研制抗蜱疫苗和生物杀螨剂

• 批准号: 15208028
• 负责人: FUJISAKE Kozo
• 金额: $ 29.54万
• 依托单位: Kagoshima University (2006)Obihiro University of Agriculture and Veterinary Medicine (2003-2005)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15208028/
• 关键词: tick; cDNA library; Tick bioactive molecules; TBM; baculovirus; recombinant; bioacaricide; anti-tick vaccine

The objective of this research is to develop bioacaricides and /or novel vaccines for tick control that do not pose environmental and health risks associated with chemical residues and can be used as an attractive alternative to chemical pesticides. In order to realize this approach, we must clarify tick bioactive molecules (TBMs) that are responsible for the blood-sucking and innate immunity in ticks and identify as the bioacaricide and/or vaccine candidates, because blood-sucking and innate immunity are important features of the hard ticks essential for survival, reproduction and pathogen transmission. Biochemical and functional analysis of these TBMs may therefore represent interesting target molecules to which novel and environmentally safety tick control could be directed.(1) During the period of this project, we could carry out about 50 novel TBMs the molecular identification and characterization researches on about 50 novel TBMs of the ixodid tick, Haemaphysalis longicornis.(2) We have tested a recombinant baculovirus expressing a chitinase gene from H. longicornis for its efficacy as a tick bioacaricide. A mixture of recombinant virus and chitinase was found to kill ticks faster than pure chitinase and recombinant virus alone.(3) We have examined the vaccine efficacy of two recombinant cement-like proteins, hlim2 and hlim3 against mice. The complementary effects of hlim2 on tick engorged body weight and hlim3 on tick attachment indicate the suitability of the 2 recombinant proteins for use in a cocktail vaccine.(4) We have immunized rabbits with a glycine-rich protein, RH50, from the tick Rhipicephalus haemaphysaloides and evaluated its vaccine potential against tick feeding. The results showed that the RH50 protein could be useful candidate for anti-tick vaccine because the low attachment rate both in the adult and nymphal ticks, and the high mortality of nymphal ticks feeding on recombinant RH50 immunized rabbits were found.

本研究的目的是开发生物杀螨剂和/或用于蜱控制的新型疫苗，这些疫苗不会造成与化学残留相关的环境和健康风险，并且可以用作化学农药的有吸引力的替代品。为了实现这一途径，我们必须阐明蜱类生物活性分子（tick biological molecules，TBMs），它们负责蜱类的吸血和先天免疫，并确定为生物杀螨剂和/或疫苗候选者，因为吸血和先天免疫是硬蜱生存、繁殖和病原体传播所必需的重要特征。因此，这些TBMs的生物化学和功能分析可能代表了新的和环境安全的蜱控制可能针对的有趣的靶分子。(1)在本项目期间，我们可以开展约50种新的蜱类抗菌物质的分子鉴定和特性研究。(2)我们已经测试了表达来自H的几丁质酶基因的重组杆状病毒。longicornis作为蜱生物杀螨剂的功效。重组病毒和几丁质酶的混合物被发现比纯几丁质酶和重组病毒单独杀死蜱更快。(3)我们研究了两种重组骨水泥样蛋白hlim 2和hlim 3对小鼠的疫苗效力。hlim 2对蜱饱食体重和hlim 3对蜱附着的互补作用表明这2种重组蛋白适用于鸡尾酒疫苗。(4)我们已经用来自蜱Rhipicephalus haemaphysaloides的富含甘氨酸的蛋白质RH 50免疫兔子，并评估其对蜱喂养的疫苗潜力。结果表明，重组蛋白RH 50在成蜱和若虫体内的粘附率均较低，且若虫对重组蛋白RH 50免疫的家兔有较高的死亡率，可作为抗蜱疫苗的候选抗原。

项目成果

Fate of GFP-expressed Escherichia coli in the midgut and reaction of hemocytes in a tick, Ornithodoros moubata (Acari : Argasidae).

中肠中 GFP 表达的大肠杆菌的命运以及蜱虫 Ornithodoros moubata（螨虫：蜱科）中血细胞的反应。

• 发表时间: 2004
• 期刊: Exp.Parasitol. 108(1-2)
• 作者: Matsuo;T.
• 通讯作者: T.

Kadota, K.: "Morphological and populational characteristics of hemocytes of Ornithodoros moubata nymphs during the ecdysial phase"J.Med.Entomol.. 40・6. 770-776 (2003)

Kadota, K.：“毛白钝缘鸟若虫蜕皮期血细胞的形态和种群特征”J.Med.Entomol.. 40・6 (2003)。

Molecular cloning and functional characterization of an aspartic protease from the hard tick Haemaphysalis longicornis

• DOI: 10.1016/j.ibmb.2005.10.003
• 发表时间: 2006-01-01
• 期刊: INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
• 影响因子: 3.8
• 作者: Boldbaatar, D;Sikasunge, CS;Fujisaki, K
• 通讯作者: Fujisaki, K

A secreted cystatin from the tick Haemaphysalis longicornis and its distinct expression patterns in relation to innate immunity.

• DOI: 10.1016/j.ibmb.2006.03.003
• 发表时间: 2006-07
• 期刊: Insect biochemistry and molecular biology
• 影响因子: 3.8
• 作者: Jinlin Zhou;M. Ueda;R. Umemiya;B. Battsetseg;D. Boldbaatar;X. Xuan;K. Fujisaki

Haemaphysalis longicornis:: Molecular characterization of a homologue of the macrophage migration inhibitory factor from the partially fed ticks

• DOI: 10.1016/j.exppara.2006.07.006
• 发表时间: 2007-02-01
• 期刊: EXPERIMENTAL PARASITOLOGY
• 影响因子: 2.1
• 作者: Umemiya, Rika;Hatta, Takeshi;Fujisaki, Kozo
• 通讯作者: Fujisaki, Kozo