Establishment of a therapeutic system in surgery for ulcerative colitis

溃疡性结肠炎外科治疗体系的建立

• 批准号: 15209041
• 负责人: SASAKI Iwao
• 金额: $ 28.37万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15209041/
• 关键词: ulcerative colitis; total proctocolectomy; intestinal adaptation; pouchitis; drug delivery system; 潰瘍性大腸炎; 治療指針; drug delivery system; 大腸全摘術; 家族性大腸腺腫癌; 家族性大腸腺腫症; 回腸襄炎

1.Analysis of mechanisms of intestinal adaptation after total proctocolectomy for ulcerative colitisComparative studies of oral and anal mucosae demonstrated that in addition to hormonal changes, fecal stream is required for full induction of the sodium transport system (which includes SGLT1-mediated transport) in the remnant ileum following total proctocolectomy. Colonic transformation is thought to be a phenotypic alteration in the ileum following total colectomy (TC) but has not been well addressed at molecular level. We demonstrated using cDNA roicroarray that altered gene expression following TC is, in part, characterized by "colon-like" transformation. Furthermore, circulating aldosterone appears to play a part of roles in altoring and/or adapting gene expression in intestinal epithelium.2.A novel approach to promote intestinal adaptationOral administration of aldosterone-PLGA microsheres successfully induced the expression of several molecules and activated sodium transport in the ileal mucosa, both of which are essential for intestinal adaptation. Pre-and/or post-operative treatment with this drug may compensate for the excessive loss of sodium and water following proctocolectomy.3.Diagnosis and treatment of pouchitis, a novel mucosal inflammationBecause endoscopic finding is a key component of this criteria, we edited pouchitis atlas. Atlas includes description of special consideration for pouch endoscopy and many photos showing a wide variety of findings and various degree of inflammation. Atlas enabled us to evaluate endoscopic findings more objectively and classify findings into normal, mild, moderate, and severe.

1.溃疡性结直肠癌全直肠切除后肠道适应机制的分析对口腔和肛门粘膜的比较研究表明，除了激素变化外，全直肠切除后残留回肠中的钠转运系统(包括SGLT1介导的转运)的充分诱导还需要粪便流动。结肠转化被认为是全结肠切除(TC)后回肠的一种表型改变，但在分子水平上尚未得到很好的解决。我们利用基因芯片技术证明了TC后基因表达的改变部分表现为“冒号样”的转化。此外，循环中的醛固酮似乎在调节和/或调节肠道上皮细胞的基因表达方面发挥了作用。2.促进肠道适应的新方法口服醛固酮-PLGA微球成功地诱导了几个分子的表达，并激活了回肠粘膜的钠转运，这两者都是肠道适应所必需的。术前和/或术后应用该药可以弥补直肠切除术后钠和水的过量丢失。3.膀胱炎的诊断和治疗，一种新的粘膜炎症由于内窥镜检查是这一标准的关键组成部分，我们编辑了膀胱炎图谱。Atlas包括对内窥镜检查的特殊考虑的描述和许多照片，显示各种各样的发现和不同程度的炎症。Atlas使我们能够更客观地评估内窥镜检查结果，并将检查结果分为正常、轻度、中度和重度。

项目成果

An approach to analyze mechanisms of intestinal adaptation following total protocolectomy

一种分析全方案切除术后肠道适应机制的方法

• 发表时间: 2006
• 期刊: J Gastrointestinal Surg. (In press)
• 作者: Fukushima K;et al.
• 通讯作者: et al.

Factors affecting the bowel function after proctocolectomy and heal J pouch anal anasiomosis for alcerative colitis

直肠结肠切除术后肠功能的影响因素及治疗溃疡性结肠炎的J储袋肛管吻合术

• 发表时间: 2006
• 期刊: J Gastrointest Surg. 10(7)
• 作者: Shibata C;Funayama Y;Fukushima K;Takahashi K;Saijo F;Nagao M;Haneda S;Watanabe K;Kudoh K;Kohyama A;Sasald I.
• 通讯作者: Sasald I.

In vivo induction of prostasin mRNA in colonic epithelial cells by dietary

通过膳食在体内诱导结肠上皮细胞中的前列腺素 mRNA

• 发表时间: 2004
• 期刊: Journal of Gastroenterology 369(10)
• 作者: Takahashi T.;K Fukushimaほか
• 通讯作者: K Fukushimaほか

H Ogawa, K Fukushima ほか: "Increased expression of HIP/PAP and regenerating gene III in human inflammatory bowel disease and a murine bacterial reconstitution model"Inflammatory Bowel Disease. 9. 162-170 (2003)

H Okawa、K Fukushima 等人：“人类炎症性肠病和小鼠细菌重建模型中 HIP/PAP 和再生基因 III 的表达增加”炎症性肠病 9. 162-170 (2003)。

K.Fukushima ほか: "Non-pathogenic bacteria modulate colonic epithelial gene expression in germ-free mice"Scand J Gastroenterol. 38. 626-634 (2003)

K. Fukushima 等人：“非致病性细菌调节无菌小鼠中的结肠上皮基因表达”Scand J Gastroenterol。 38. 626-634 (2003)