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Development of a waveguide surface plasmon resonance biosensor fabricated by a polymer replication method

聚合物复制法制造的波导表面等离子共振生物传感器的开发

基本信息

批准号：
16201030
负责人：
AOYAMA Shigeru
金额：
$ 32.36万
依托单位：
Corporate R&D Headquarters, OMRON Corporation (2006-2007)Osaka University (2004-2005)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2007
项目状态：
已结题

项目摘要

Biosensors are currently used for the purpose of detecting protein-protein interactions. However, this sensor can be operated by experts who require an expensive and complicated optical system with unique technology at the laboratory level. Therefore, recently a waveguide surface plasmon resonance (WSPR) sensor that can integrate various optical functions has attracted attention. Moreover, we are developing imprint technology that can inexpensively produce fine structure with high precision.In this research, a WSPR sensor was fabricated by polymer utilizing imprint technology. Compact and simple composition was enabled by changing a complicated optical system into an optical integrated circuit and integration on a plate using this imprint technology. A technique to transfer this integrated circuit on a glass substrate with imprint technology was developed, proving that an optical integrated circuit can be economically manufactured. Furthermore, a functional membrane for capturing a target protein to a sensor chip was developed. Improvements were also realized in the absolute quantity of target protein capture by the high-density of a functional membrane and in the rate that only catches a specific protein by improving the molecular bond structure of a functional membrane. Moreover, to prove its usefulness as a biosensor, the optimal structure design of a sensor chip was achieved, a sensor chip was produced using imprint technology, and the adsorption reaction of the model protein was detected using the sensor chip. The refractive-index resolution was 8.9x10^<-5> [RIU (refractive index unit)].

生物传感器目前用于检测蛋白质-蛋白质相互作用的目的。然而，这种传感器可以由需要昂贵且复杂的光学系统的专家在实验室水平上操作。因此，近来，可以集成各种光学功能的波导表面等离子体共振（WSPR）传感器引起了关注。此外，我们正在开发的压印技术，可以廉价地生产精细结构和高精度。在本研究中，WSPR传感器的聚合物利用压印技术制造。通过使用该压印技术将复杂的光学系统改变成光学集成电路并集成在板上，能够实现紧凑且简单的组成。开发了利用压印技术将该集成电路转移到玻璃基板上的技术，证明了可以经济地制造光学集成电路。此外，开发了用于将目标蛋白捕获到传感器芯片的功能膜。通过功能膜的高密度，也实现了靶蛋白捕获的绝对量的提高，并且通过改善功能膜的分子键结构，实现了仅捕获特定蛋白的速率的提高。此外，为了证明其作为生物传感器的有用性，实现了传感器芯片的最佳结构设计，使用压印技术制造了传感器芯片，并使用传感器芯片检测了模型蛋白质的吸附反应。折射率分辨率为8.9x10^<-5>[RIU（折射率单位）]。

项目成果

期刊论文数量（40）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

表面プラズモンセンサ

表面等离子体传感器

DOI：
发表时间：
2006
期刊：
影响因子：
0
作者：
通讯作者：

Development of a single-mode waveguide SPR sensor fabricated by a polymer replication method

聚合物复制法制备单模波导SPR传感器的开发

DOI：
发表时间：
2005
期刊：
Extended Abstracts (The 52nd Spring Meeting) ; The Japan Society of Applied Physics and Related Societies No.3
影响因子：
0
作者：
J.Kishimoto;T.Nishikawa;T.Matsushita;H.Sezaki;H.Yamashita;S.Norioka;T.Wazawa;S.Aoyama
通讯作者：
S.Aoyama

Total internal reflection fluorescence microscopy in single molecule nanobioscience