Development of a waveguide surface plasmon resonance biosensor fabricated by a polymer replication method

点击翻译按钮获取中文摘要

Biosensors are currently used for the purpose of detecting protein-protein interactions. However, this sensor can be operated by experts who require an expensive and complicated optical system with unique technology at the laboratory level. Therefore, recently a waveguide surface plasmon resonance (WSPR) sensor that can integrate various optical functions has attracted attention. Moreover, we are developing imprint technology that can inexpensively produce fine structure with high precision.In this research, a WSPR sensor was fabricated by polymer utilizing imprint technology. Compact and simple composition was enabled by changing a complicated optical system into an optical integrated circuit and integration on a plate using this imprint technology. A technique to transfer this integrated circuit on a glass substrate with imprint technology was developed, proving that an optical integrated circuit can be economically manufactured. Furthermore, a functional membrane for capturing a target protein to a sensor chip was developed. Improvements were also realized in the absolute quantity of target protein capture by the high-density of a functional membrane and in the rate that only catches a specific protein by improving the molecular bond structure of a functional membrane. Moreover, to prove its usefulness as a biosensor, the optimal structure design of a sensor chip was achieved, a sensor chip was produced using imprint technology, and the adsorption reaction of the model protein was detected using the sensor chip. The refractive-index resolution was 8.9x10^<-5> [RIU (refractive index unit)].