Interaction between Metallome and Proteome in the Marine Ecosystem

1.化学形态分析和生物活性痕量金属原位测定的进展(1)悬浮颗粒物Co、Ni、Cu、Zn、Cd和Pb的测定；(2)溶解和酸溶的Zr、Hf、Nb、Ta和W的测定；(3)碱度和总碳酸盐的原位流动分析；(2)浮游植物和细菌的酶和蛋白质分析方法的发展；(1)光合作用系统中Fe-S蛋白PSAC和PSBA(D1)的检测。(2)抗铁还蛋白和抗黄毒素抗体，它们分别与海洋硅藻的铁还蛋白和黄还蛋白发生交叉反应，以估计它们的铁胁迫。3.金属组和蛋白质组的变化及其关系的观察(1)苏鲁海、南中国海和太平洋之间海水中Fe、Co、Ni、Cu、Zn和Cd的不同分布。(2)在第二次亚北极北太平洋(SEED-II)中尺度铁富集实验中，研究了富铁对浮游植物群落结构和生物活性痕量金属形态的影响。(3)在光合作用蛋白质分析的基础上，海水中的Fe已被证明是控制奥亚索地区硅藻春季水华的主要因素。(4)研究了Suruga湾纳米鞭毛虫和纤毛虫生物量的日动态。(5)证实了微量金属对微生物胞外酶活性的影响。4.浮游植物对培养物中金属的吸收机理研究(1)浮游植物培养基中铁的尺寸分布。用LC-MS分析了浮游植物渗出的铁配体。(2)分析了赤潮浮游植物在缺铁条件下形成的膜蛋白。

1.Development of chemical speciation and in situ determination of bioactive trace metals(1)Determination of suspended particulate Co, Ni, Cu, Zn, Cd and Pb has been developed.(2)Determination of dissolved and acid-dissolvable Zr, Hf, Nb, Ta and W has been developed.(3)In-situ flow analysis of alkalinity and total carbonate has been developed.2.Development of analytical methods for enzymes and proteins of phytoplankton and bacteria(1)Detection of Fe-S protein PsaC and PsbA(D1) in photosynthetic system has been developed.(2)Anti-ferredoxin and anti-flavodoxin antibodies, which cross-react with ferredoxin and flavodoxin of marine diatoms, respectively, have been developed using synthetic peptides in order to estimate their Fe stress.3.Observation of the variation and relationship of metallome and proteome(1)Different distributions of Fe, Co, Ni, Cu, Zn and Cd in seawater between the Sulu Sea, South China Sea and Pacific Ocean were observed.(2)During the second mesoscale iron enrichment experiments in the western subarctic North Pacific (SEEDS-II), the effects of iron enrichment on the phytoplankton community structure and the speciation of bioactive trace metals were examined.(3)On the basis of analysis of photosynthetic proteins, Fe in seawater has been proved to be a major factor controlling the spring bloom of diatoms in the Oyashio region.(4)Diurnal dynamics of biomass of nano-flagellates and ciliates in Suruga Bay was investigated.(5)The effects of trace metals on the activity of extracellular enzymes of microbes have been proved.4.Study on metal uptake mechanism of phytoplankton in culture(1) The size distribution of Fe in culture media of phytoplankton was investigated. Fe ligands exuded from phytoplankton were analyzed by LC-MS.(2)Membrane proteins revealed in red tide forming phytoplankton Prymnesium parvum under Fe deficiency were analyzed.