Molecular mechanism for adsorption and adsorption of soil components formed on the surface of equipment wall during manufacturing and development of an efficient cleaning method

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In various manufacturing processes, cleaning of the equipment and piping systems used for manufacturing of various products is requisite for maintaining sanitary conditions and/or functions of the equipments. In particular, in the food- and bio-products manufacturing processes, a large amount of energy and detergents are usually consumed in addition to time, since soils or residues of the products strongly interact with the equipment surface. In this study, we studied adsorption mechanisms of various proteins/peptides and low-molecular weight substances as a model soil component on the surfaces of various metal oxides, plastics, and glasses. We clarified the molecular mechanisms for reversible/irreversible adsorptions, states and orientation of the molecules adsorbed, the effects of the pI values of the metal oxide surfaces/solution pHs on the adsorption characteristics, and conformational change of the adsorbed proteins, on the basis of adsorption/desorption experiments, IR spectrum analyses, and molecular calculations. Furthermore, we investigated the effects of various factors such as the kinds of the co-existing salts and their concentrations on the removal rates of the metal oxide surface fouled with proteins, using H2O2-electrolysis cleaning method that was developed by the author's group. Furthermore, we developed a method to immobilize proteins with controlled orientation and structure for various uses in chip-based biotechnology, which was derived in the course of investigation on the adsorption mechanism of proteins.