Molecular mechanism for adsorption and adsorption of soil components formed on the surface of equipment wall during manufacturing and development of an efficient cleaning method

制造过程中设备壁表面形成的土壤成分吸附吸附的分子机理及高效清洗方法的开发

• 批准号: 16206076
• 负责人: NAKANISHI Kazuhiro
• 金额: $ 29.95万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16206076/
• 关键词: adsorption; adsorption equilibrium; protein; Peptide; metal oxide surface; plastic surface; cleaning; hydrogen peroxide-electrolysis cleaning; 表面プラズモン共鳴; 脂肪酸・油脂の付着; 親和性ペプチド; 付着機構; 吸着等温線; β-ラクトグロブリン; 脱離速度; ポリスチレン; 付着; 固体表面; アフィニティペプチド; 疎

In various manufacturing processes, cleaning of the equipment and piping systems used for manufacturing of various products is requisite for maintaining sanitary conditions and/or functions of the equipments. In particular, in the food- and bio-products manufacturing processes, a large amount of energy and detergents are usually consumed in addition to time, since soils or residues of the products strongly interact with the equipment surface. In this study, we studied adsorption mechanisms of various proteins/peptides and low-molecular weight substances as a model soil component on the surfaces of various metal oxides, plastics, and glasses. We clarified the molecular mechanisms for reversible/irreversible adsorptions, states and orientation of the molecules adsorbed, the effects of the pI values of the metal oxide surfaces/solution pHs on the adsorption characteristics, and conformational change of the adsorbed proteins, on the basis of adsorption/desorption experiments, IR spectrum analyses, and molecular calculations. Furthermore, we investigated the effects of various factors such as the kinds of the co-existing salts and their concentrations on the removal rates of the metal oxide surface fouled with proteins, using H2O2-electrolysis cleaning method that was developed by the author's group. Furthermore, we developed a method to immobilize proteins with controlled orientation and structure for various uses in chip-based biotechnology, which was derived in the course of investigation on the adsorption mechanism of proteins.

在各种制造过程中，用于制造各种产品的设备和管道系统的清洁对于保持设备的卫生条件和/或功能是必需的。特别地，在食品和生物制品的制造过程中，除了时间之外，通常还消耗大量的能量和清洁剂，因为产品的污垢或残留物与设备表面强烈地相互作用。在这项研究中，我们研究了各种蛋白质/肽和低分子量物质作为模型土壤成分在各种金属氧化物，塑料和玻璃表面上的吸附机制。通过吸附/脱附实验、红外光谱分析和分子计算，阐明了可逆/不可逆吸附的分子机理、吸附分子的状态和取向、金属氧化物表面pI值/溶液pH值对吸附特性的影响以及吸附蛋白质的构象变化。此外，我们研究了各种因素，如共存的盐的种类和它们的浓度对金属氧化物表面的蛋白质污染的去除率的影响，使用由作者的小组开发的H2 O2-电解清洗方法。此外，我们开发了一种方法，以控制蛋白质的取向和结构，用于基于芯片的生物技术中的各种用途，这是在蛋白质的吸附机理的研究过程中得出的。

项目成果

Fouling characteristics of soils composed of tannic acid-proteins and their cleaning characteristics by H202 electrolysis treatment

单宁酸-蛋白质土壤的污染特性及其H2O2电解处理的净化特性

• 发表时间: 2005
• 作者: K.;Imamura;Y.;Aramoto;H.;Imanaka;T.;Sakiyama;T.;Takahashi;K.;Nakanishi
• 通讯作者: Nakanishi

ポリスチレン表面に親和性を示すペプチドタグを付加した酵素の設計

带有肽标签的酶的设计，显示出对聚苯乙烯表面的亲和力

• 发表时间: 2005
• 作者: Oyabu;S. ; Yun;Min S. ; Murayama;T. ; Sanders;D.B. ; Kawara;K. ; Taniguchi;Y. ; Veilleux;S. ; Okuda;H. ; _Matsuhara;H. ; Cowie;L.L. ; Sato;Y. ; Wakamatsu;K.;Kiyotomi Kaneda;今石大輔
• 通讯作者: 今石大輔

Controll of protein orientation on the metal oxide surfaces

金属氧化物表面蛋白质取向的控制

• 发表时间: 2006
• 作者: K.;Yanagata;M.;Nakazumi;H.;Imanaka;K.;Imamura;K.;Nakanishi
• 通讯作者: Nakanishi

金属表面高親和性ペプチドを用いたタンパク質付着特性制御

使用对金属表面具有高亲和力的肽控制蛋白质粘附特性

• 发表时间: 2006
• 作者: Matsuhara;H. ; Murakami;H. ; Nakagawa;T. ; Wada;T. ; Matsuma;S. ; Oyabu;S. ; Takagi;T. ; Pearson;C. P. ; Kaneda;H. ; Usui;F. ; Shirahata;M. ; Shibai;H. ; Kawada;M. ; Onaka;T. ; Doi;K.Omagari et al.;Y.Konda et al.;金田 清臣;F.A. Nae;柳田圭介
• 通讯作者: 柳田圭介

タンパク質の固体表面への付着機構と機能制御-タンパク性汚れの付着・洗浄からイムノアッセイまで-

蛋白质附着在固体表面的机制和功能控制 - 从蛋白质污渍的附着和清洁到免疫测定 -

• 发表时间: 2006
• 作者: Kuniyuki Omagariand;Saburo Matunaga;井筒直樹;中西一弘
• 通讯作者: 中西一弘