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Investigation of functional rhizo-biocomplex on highly adverse soil-adapting plants and application of the functional rhizo-biocomplex to agro-technology.

功能性根际生物复合物对高度不利的土壤适应植物的研究及其在农业技术中的应用。

基本信息

批准号：
16208032
负责人：
HASHIDOKO Yasuyuki
金额：
$ 32.28万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2007
项目状态：
已结题

项目摘要

Using local peat soil from Central Kalimantan, Indonesia, a saprophytic soil fungus Penicillium sp. Isolate Y-1 from X. complanata seedbed was tested its seed-germination promotion activity. The seeds germinated well by means of inoculation with Y-1 fungus, but the resulting seedlings were all died after one month-incubation in the local peat soil bed. Only when the supernatant of the local soil suspension without sterilization was further inoculated to the seedbeds, the germinated seedlings that had been inoculated with Y-1 resulted in survival and normal growth of the seedlings over 6 month. This suggested that saprophytic fungi stimulate seed germination, while some soil bacteria provide nutrients to the germinated seedlings. On the other hand, in Siberian Taiga forest bed soil, oligotrophic Burkholderia and Pseudomonas occupied soil microflora, and they showed a relatively high acetylene reduction activity at 10-15℃,under a lower concentration of carbon source (0.02-0.05%). Including medium pH, these soil bacteria were highly adapted to culture conditions agreeable to the permafrost soil, so that physicochemical conditions similar to activated permafrost soil in summer seasons resulted in relatively higher nitrogen-fixing activity. Moreover, gellan gum as gel matrix allowed the bacteria to show maximal nitrogen-fixing potential. Under a field condition of newly accumulated volcanic ash soil accumulated extreme soil land in Mt. Usu, Hokkaido, the land allowed pioneer plants to possess mycorrhizal symbionts and grew dominantly. Particularly, those of willow tree saplings constitutively possessed ectomycorrhizal fungi, while herbivorous weeds, except horsetail and rasp, allowed symbiosis with arbuscular mycorrhizal fungi. Thus, it was found that pioneer plants distributing throughout fresh volcanic ash soil in Mt. Usu were dependent on mycorrhizal infection.

利用印度尼西亚加里曼丹中部的泥炭土，从X.对沙苑子苗床进行了种子萌发促进活性的测定。用Y-1真菌接种种子，种子萌发良好，但在当地泥炭土床中培养一个月后，所产生的幼苗全部死亡。只有当当地土壤悬浮液的上清液未经灭菌进一步接种到苗床上时，接种Y-1的发芽苗才能存活并正常生长6个月以上。这表明腐殖真菌刺激种子萌发，而一些土壤细菌为发芽的幼苗提供营养。而在西伯利亚针叶林床土中，贫营养的伯克霍尔德菌和假单胞菌占据着土壤微生物区系，在10-15℃、较低碳源浓度（0.02-0.05%）下，它们表现出较高的乙炔还原活性。包括介质pH值，这些土壤细菌高度适应培养条件的冻土，使物理化学条件类似于激活的冻土在夏季季节导致相对较高的固氮活性。此外，结冷胶作为凝胶基质允许细菌显示最大的固氮潜力。在新积火山灰土壤的野外条件下，研究了火山灰积极土的形成机理.北海道的乌苏，这片土地允许先锋植物拥有菌根共生体并占优势。特别是杨柳幼苗的外生菌根真菌组成，而草食性杂草，除了马尾草和锉刀，允许与丛枝菌根真菌共生。因此，我们发现，先锋植物分布在整个新鲜的火山灰土壤在山。Usu依赖于菌根侵染。

项目成果

期刊论文数量（91）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Isolation and identification of potential phosphatase solubilizing bacteria from the rhizoplane of Oryza sativa L. cv. BR29 of Bangladesh

水稻根面潜在磷酸酶溶解细菌的分离和鉴定

DOI：
发表时间：
2007
期刊：
Zeitschrift fur Naturforschung 62c
影响因子：
0
作者：
M.T.;Islam,A.;Deora,Y.;Hashidoko,A.;Rahman,T.;Ito;S.;Tahara
通讯作者：
Tahara

Screening of rhizobacteria from dipterocarp youngsters for promotion of early growth of Shorea selanica seedlings

龙脑香幼苗根际细菌的筛选促进龙脑香幼苗早期生长

DOI：
发表时间：
2007
期刊：
Tropics 16
影响因子：
0
作者：
I.R.;Sitepu,Aryanto,N.;Ogita,M.;Osaki,E.;Santoso,S.;Tahara,Y.;Hashidoko
通讯作者：
Hashidoko

Mycorrhizal colonization status of plant species established in an exposed area following the 2000 eruption of Mt. Usu, Hokkaido, Japan

2000 年日本北海道有珠山喷发后暴露区域植物物种的菌根定植状况

DOI：
发表时间：
2008
期刊：
Landscape and Ecological Engineering
影响因子：
2
作者：
K.;Obase;Y.;Tamai;T.;Yajima;T.;Miyamoto
通讯作者：
Miyamoto

Effects of agricultural land-use change and forest fire on N2O emission from tropical peatlands, Central Kalimantan, Indonesia

DOI：
10.1111/j.1747-0765.2006.00084.x
发表时间：
2006-10
期刊：
Soil Science and Plant Nutrition
影响因子：
2
作者：
F. Takakai;T. Morishita;Y. Hashidoko;U. Darung;Kanta Kuramochi;S. Dohong;S. Limin;R. Hatano
通讯作者：
F. Takakai;T. Morishita;Y. Hashidoko;U. Darung;Kanta Kuramochi;S. Dohong;S. Limin;R. Hatano

カフェ酸脱炭酸活性をもつ根面細菌の検索とその脱炭酸酵素遺伝子解析による機能性解明

寻找具有咖啡酸脱羧活性的根际细菌并通过脱羧酶基因分析阐明其功能