Effect of diverse nitrogen-fixing bacterial community inhabiting the rhizoplane of acid sulfate soil-tolerant plants on nitrogen circulation around the rhizophere.

耐酸性硫酸盐土壤植物根面的多种固氮细菌群落对根际周围氮循环的影响。

• 批准号: 13460143
• 负责人: HASHIDOKO Yasuyuki
• 金额: $ 9.22万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13460143/
• 关键词: acid sulfate soil-tolerant plants; rhizoplane bacteria; nitrogen fixing bacteria; nitrogen circulation; Sphingomonas sp.; rhizo biocomplex; functionality on the rhizoplane; nifgenes; 強酸性耐性; 根面微生物; 微生物フローラの多様性; 単生窒素固定細菌; 根面pHの上昇; Sphingomonas

In this study, we cultured rot washings in nitrogen-free, soft gel medium as a primary screening of functional rhizoplane and/or rhizosphere microbes, and we efficiently and selectively obtained α- and β-proteobacteria to exclude saprophytic γ-proteobacteria. Those oligotrophic and/or diazotrophic bacteria were visualized their behaviors in the soft gel medium, including their biocomplex formation and tripartite partnerships. The bacterial flora propagated in the N-free soft gel medium of gellan gum base survived relatively longer in the medium at room temperature due to a biofilm-like colonization and quorum-sensing like dormancy. Thus, we were able to trail new area of functional rhizo-biocomplex connected to the study of behabior of rhizoplane microfloral communities. As a demonstration, we have isolated and sub-cultured two rhizoplane bacteria, Sphingomonas rosa and Burkholderia cepacia, from rhizoplane of Melastoma malabathricum, a representative acid sulfate soil-tolerant plant, and identified them with 16S rRNA gene sequencing and data base search. The S. rosa showed. abilities to alter rhizospherous conditions and also to enhance nitrogen supply from the rhizo-biocomplex in a sotrongly acidic conditions. Like S. rosa, many other Sphingomonas spp. often isolated from the rhizoplane of acid-tolerant plants in Kalimantan had characteristics of 1) contributing to reliving divert soil in the rhizosphere, 2) fixing nitrogen in the rhizoplane or at least adjusting conditions for nitrogen fixation, and 3) being regulated the microfloral population via Mg^<2+> in the rhizosphere. In addition, Sphingomonas spp. had tight partnerships and tripartite-like co-works in the rhizoplane and/or inner tissues of host plants. Sphingomonas spp. are thus expected to be a motive force for nitrogen circulation and high genetic diversity in the rhizo-biocomplex.

本研究采用无氮软凝胶培养基培养腐烂洗液，初步筛选出具有功能的根面和/或根际微生物，并高效、选择性地获得α-和β-变形菌，排除了腐殖化的γ-变形菌。这些贫营养和/或固氮细菌在软凝胶介质中的行为，包括它们的生物复合物的形成和三方伙伴关系。在结冷胶基无氮软凝胶培养基中繁殖的细菌植物群在室温下存活时间较长，这是由于其具有生物膜样定殖和群体感应样休眠。因此，我们能够探索新的领域的功能根生物复合体连接到根际微植物群落的行为的研究。作为示范，我们从耐酸性硫酸盐土壤的代表性植物马拉巴木根面中分离并传代培养了2株根面细菌，即玫瑰鞘氨醇单胞菌和洋葱伯克霍尔德菌，并通过16 SrRNA基因测序和数据库检索对其进行了鉴定。色葡萄罗莎表示。改变根际条件的能力，并在强酸性条件下提高根生物复合体的氮供应。就像S。rosa，许多其它鞘氨醇单胞菌属（Sphingomonaspp.）通常从加里曼丹耐酸性植物的根面分离的细菌具有以下特征：1）有助于根际土壤的恢复，2）在根际固定氮或至少调节氮固定的条件，3）通过Mg^2+在根际调节微植物群。此外，鞘氨醇单胞菌属。在寄主植物的根面和/或内部组织中具有紧密的伙伴关系和三方合作。鞘氨醇单胞菌属因此，预计是一个动力的氮循环和高遗传多样性的根生物复合体。

项目成果

Jun Wasaki: "Secreted acid phosphatase is expressed in cluster roots of Lupin in response to phosphorus deficiency"Plant and Soil. 248. 129-136 (2003)

Jun Wasaki：“分泌的酸性磷酸酶在羽扇豆丛根中表达，以应对磷缺乏”植物和土壤。

Soft Gel Medium Solidified with Gellan Gum for Preliminary Screening for Root-associating, Free-living Nitrogen-fixing Bacteria Inhabiting the Rhizoplane of Plants

• DOI: 10.1271/bbb.66.2259
• 发表时间: 2002-01
• 期刊: Bioscience, Biotechnology, and Biochemistry
• 作者: Y. Hashidoko;Motohiko Tada;M. Osaki;S. Tahara

橋床泰之: "二次代謝産物を介した高等植物と着生微生物の相互作用研究"日本農芸化学会誌. 78巻. 1210-1217 (2003)

Yasuyuki Hashidoko：“次生代谢物介导的高等植物与附生微生物之间的相互作用的研究”日本农业化学学会杂志第78卷1210-1217（2003年）。

Localization of flavonoids in the yellow lupin seedlings and their UV-B-absorbing potential.

黄色羽扇豆幼苗中类黄酮的定位及其 UV-B 吸收潜力。

• 发表时间: 2002
• 期刊: Zeitschrift fur Naturforschung 57c
• 作者: Yasufumi Katagiri;Yasuyuki Haskidoko;Satoshi Tahara.
• 通讯作者: Satoshi Tahara.

Penicillium fungi from Picea glehnii seeds protect the seedlings from damping-off.

云杉种子中的青霉菌可以保护幼苗免遭立枯病。

• 发表时间: 2001
• 期刊: New Phytologists 152
• 作者: Keiko Yamaji;Yukiharu Fukushi;Yasuyuki Hashidoko;Tadashi Yoshida;Satoshi Tahara.
• 通讯作者: Satoshi Tahara.