Correlation between diversity of nitrogen-fixing bacterial community inhabiting the rhizoplane of acid sulfate soil-tolerant local paddy plant and rice production

耐酸性硫酸土水稻根面固氮细菌群落多样性与水稻产量的相关性

• 批准号: 13575031
• 负责人: HASHIDOKO Yasuyuki
• 金额: $ 6.78万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13575031/
• 关键词: acid sulfate soil-tolerant rice; rhizoplane bacteria; nitroren-fixing bacteria; low-in put sustainable agriculture; Sphingomonas sp.; rhizoplane pH; local rice; traditinal agriculture; 強酸性耐性イネ; 内生微生物とスウォーミング; 微生物フローラの多様性; 単生窒素固定細菌; 根の機能性; ローカ

In this study, we revealed a potent ability of Kalimantan local rice varieties cultivated on acid sulfate soil paddocks in South Kalimantan, Indonesia, and this rice production is regarded as a representative highly produced low-input sustainable agriculture. From the rhizoplane of local rice varieties, we have often isolated Sphingomonas spp. and a major strain was the same with those isolated from Melastoma malabathricum rhizoplane and Xyris complanata rhizosphere in South and Central Kalimantans. Thus, many Sphingomonas spp. isolated from the local rice varieties were considered to be functional rhizobacteria, 1) contributing to moderation of the rhizospherous environments, 2) directly fixing nitrogen or, at least, adjust the rhizoplane condition to be more suitable for nitrogen fixation, and 3) playing an important role in rhizo-biocomplex formation due to acceleration of microbial biomass accumulation. Therefore, rhizospherous microfloral communities including the Sphingomonas sp. established along the root system of local rice varieties are able to acquire nitrogen and buffer the rhizospherous environments, of which potent abilities are coming from their genetic diversity and stability. Since aerenchyma of the local paddy rice are remarkably developed well in the stems and roots, it is further necessary to study relationship between the inner tissue structures and endophytic nitrogen fixation.

在这项研究中，我们揭示了一个强大的能力，加里曼丹当地水稻品种种植在酸性硫酸盐土壤围场在南加里曼丹，印度尼西亚，这种水稻生产被认为是一个代表性的高产低投入的可持续农业。从当地水稻品种的根面，我们经常分离到鞘氨醇单胞菌属。从加里曼丹南部和中部地区的M.malabathricum根际和Xyris complanata根际中分离到的菌株为优势菌株。因此，许多鞘氨醇单胞菌属（Sphingomonasspp.）从当地水稻品种中分离到的10种根际细菌被认为是功能性根际细菌，1）有助于调节根际环境，2）直接固氮或至少调节根面条件使其更适合于固氮，以及3）由于加速微生物量积累而在根生物复合体的形成中起重要作用。因此，包括鞘氨醇单胞菌在内的根际微区系具有沿着分布于水稻品种根际的氮素吸收和根际环境缓冲能力，其强大的能力来源于其遗传多样性和稳定性。由于当地水稻茎和根的通气组织非常发达，因此有必要进一步研究其内部组织结构与内生固氮的关系。

项目成果

Yasuyuki Hashidoko: "Rhizoplane pH and rhizoplane microflora of local rice varieties grown on acid sulfate soil in South Kalimantan"Tropics (Proceedings of TROPEAT 2002). (Accepted for publication). (2003)

Yasuyuki Hashidoko：“南加里曼丹酸性硫酸盐土壤上生长的当地水稻品种的根面 pH 值和根面微生物区系”热带地区（TROPEAT 会议记录，2002 年）。

Yasuyuki Hashidoko, Toshihiro Hasegawa, Erry Purnomo, Suwido H.Limin, Mitsuru Osaki, Satoshi Tahara: "Rhizoplane pH of Kalimantan local paddy rice constant to neutral regions"TROPICS. (Accepted). (2004)

Yasuyuki Hashidoko、Toshihiro Hasekawa、Eerry Purnomo、Suwido H.Limin、Mitsuru Osaki、Satoshi Tahara：“加里曼丹当地水稻的根平面 pH 值恒定为中性区域”TROPICS。

Effects of genotypes and transplating methods on panicle weights of rice grown on acid sulphate soil in South Kalimantan

基因型和移栽方法对南加里曼丹酸性硫酸盐土壤水稻穗重的影响

• 发表时间: 2004
• 期刊: Japanese Journal of Crop Sciences 73
• 作者: Toshihiro Hasegawa;Erry Purnomo;Yasuyuki Hashidoko;Mitsuru Osaki;Gusty Rusmayadi.
• 通讯作者: Gusty Rusmayadi.

Yasuyuki Hashidoko, Toshihiro Hasegawa, Erry Purnomo, Mitsuru Osaki: "Rhizoplane pH and Rhizoplane Microflora of Local Rice Varieties Grown on Acid Sulfate Soil in South Kalimantan"Proceedings of International Sympodium on Land Managemen and Biodiversity

Yasuyuki Hashidoko、Toshihiro Hasekawa、Eerry Purnomo、Mitsuru Osaki：“南加里曼丹酸性硫酸盐土壤上生长的当地水稻品种的根面 pH 值和根面微生物区系”国际土地管理和生物多样性研讨会论文集

Yasuyuki Hashidoko: "Characterization of five phyllosphere bacteria isolated from Rosa rugosa leaves, and their phenotypic and metabolic properties"Bioscience, Biotechnology and Bioscience. 66・11. 2474-2478 (2002)

Yasuyuki Hashidoko：“从蔷薇叶中分离出的五种叶际细菌的特征及其表型和代谢特性”《生物科学、生物技术和生物科学》66·11（2002）。