Complete understanding of the nitrogen missing link in boreal forest ecosystems and evaluation of nitrogen provision and loss

全面了解北方森林生态系统中氮缺失环节并评估氮供应和损失

• 批准号: 20255002
• 负责人: HASHIDOKO Yasuyuki
• 金额: $ 29.2万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20255002/
• 关键词: 北方森林; 高緯度森林限界; 窒素ミッシングリンク; Spodosol; 低温耐性土壌細菌; フェザーモス; コンソーシアム形成; カンパ林; シアノバクテリア共生; VBNC; 窒素固定; 亜酸化窒素; 森林限界; ツンドラ土壌; シアノバクテリア; 林床蘇類; ヒース; カンバ林; 北方森林限界; ポドソル土壌; 森林火災; リン酸供給; 林床蘚類; 担子菌; メタンガス放出; ミズゴケ; 脱窒; 永久凍土帯; 窒素収支バランス; リン・窒素バ

Nitrogen imbalance of the boreal forest ecosystem has long been a mystery, called missing link of nitrogen in boreal forest. We challenged to this mystery, to practically understand the effects of climate change on forest biomass productivity in several types of boreal forests. Precise investigation of optimal culture conditions including compositions of the medium were obviously close to the reported conditions of the forest bed soil in summer seasons. The 16S rRNA gene-targeting DGGE analysis combined with gellan gum medium revealed the presence of Dugnella (class y-Proteobacteria) and Clostridiumin (phylum Firmicutes) in the A-horizon of the subarctic tundra soil, and also showed the aboveground vegetation affect on bacterial activity in soil. Thus, it was strongly suggested that scale of the elemental circulation in the boreal forests near the forest limit highly regulates acetylene reducing activity of free-living nitrogen-fixing bacteria. Also it was speculated that the low acetylene reduction in the forest bed soil would be highly regulated as corroborative events together with ericoid mycorrhizal and/or ectomycorrhizal fungi, leading to effective nitrogen supply to the forest ecosystem.

北方森林生态系统氮素失衡一直是个谜，被称为北方森林氮素缺失环节。我们挑战这个谜，实际上了解气候变化对森林生物量生产力的影响，在几种类型的北方森林。对最佳培养条件包括培养基组成的精确调查，与报道的夏季森林床土条件明显接近。结合结冷胶培养基的16 S rRNA基因靶向DGGE分析揭示了亚北极冻原土壤A层中存在Dugnella（Y类变形菌门）和Clostridiumin（厚壁菌门），也显示了地上植被对土壤细菌活性的影响。因此，有人强烈建议，在森林的限制附近的北方森林的元素循环的规模高度调节乙炔还原活性的自由生活的固氮细菌。此外，据推测，在森林床土壤中的低乙炔还原将高度调节作为佐证事件与杜鹃花菌根和/或外生菌根真菌，导致有效的氮供应到森林生态系统。

项目成果

A chronosequence approach detecting revegetation patterns after Sphagnum-peat mining, northern Japan.

日本北部泥炭藓开采后的植被恢复模式的时序方法。

• 发表时间: 2009
• 期刊: Ecological Research 24
• 作者: A.Nishimura;S.Tsuyuzaki;A.Haraguchi
• 通讯作者: A.Haraguchi

Buried charcoal layer and ectomycorrhizae cooperatively promote the growth of Larix gmelinii seedlings

• DOI: 10.1007/s11104-009-0040-z
• 发表时间: 2010-02
• 期刊: Plant and Soil
• 影响因子: 4.9
• 作者: Kobayashi Makoto;Yutaka Tamai;Yong Suk Kim;Takayoshi Koike

東シベリア永久凍土上の活動層から分離した土壌窒素固定細菌の特性

东西伯利亚多年冻土活动层土壤固氮细菌的特征

• 发表时间: 2009
• 作者: 石森一;小林達夫;大木洋;岡田寛;清水勇介;谷本盛光;堀口華奈;Kei Funano;原新太郎
• 通讯作者: 原新太郎

寒天粉末に含まれるフランカルボン酸誘導体は土壌細菌の活動を制限する

琼脂粉中含有的糠酸衍生物限制土壤细菌的活性

• 发表时间: 2011
• 作者: 原新太郎;橋床泰之
• 通讯作者: 橋床泰之

chronosequence approach detecting revegetation patterns after Sphagnum-peat mining, northern Japan

日本北部泥炭藓开采后的植被恢复模式的时序方法

• 发表时间: 2009
• 期刊: Ecological Research
• 影响因子: 2
• 作者: A. Nishimura;S. Tsuyuzaki;A. Haraguchi
• 通讯作者: A. Haraguchi