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Bystander effects induced with inner shell ionization followed by Auger effects

内壳电离引起的旁观者效应，随后是俄歇效应

基本信息

批准号：
17201016
负责人：
KOBAYASHI Katsumi
金额：
$ 32.03万
依托单位：
High Energy Accelerator Research Organization
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17201016/
关键词：
Environmental radiation Synchrotron X-ray X-ray microbeam Biological effects of low dose radiation Inner shell ionization Auger effect 環境放射線マイクロビーム生物物理

项目摘要

In low dose region where most of the irradiated cells can survive, biological response to radiation is now known to appear as a result of intra-and/or inter-cell signaling pathway. Purpose of this research project is to investigate the outbreak of original signal induced with energy deposited by radiation from energetic point of view. In order to utilize the tenability of synchrotron X-rays in the microbeain cell irradiation system which had been developed in our previous project funded by scientific research (A) of MIST, we developed an energy-tunable microbeam X-ray irradiation system by aligning all the components along the horizontal beam axis. After overcoming some problems concerning cell irradiation chamber, we succeeded in getting reliable and reproducible biological results. Although these are still preliminary due to the shortage of the beamtime, results are summarized as follows.1. When irradiated with X-rays tuned to Fe K-shell absorption edge, Fe-incorporated cells showed lower bystander cell death than irradiated with below-edge energy X-rays.2. When irradiated with soft X-rays tuned to S K-edge absorption edge, irradiated cells showed less sensitive than cells irradiated with below-edge X-rays. As for the bystander cell death, S K-edge X-rays showed higher lethality than below-edge X-rays.We will continue to study the mechanisms leading to interesting biological phenomena obtained as above.

在低剂量区，大多数受辐射细胞可以存活，目前已知对辐射的生物反应是由于细胞内和/或细胞间信号通路而出现的。本研究的目的是从能量的角度研究辐射沉积能量引起的原始信号爆发。为了充分利用同步加速器x射线在微束细胞辐照系统中的可行性，我们开发了一种能量可调的微束x射线辐照系统，该系统是由中国科学院科学院科研项目(A)资助的。在克服了细胞辐照室的一些问题后，我们成功地获得了可靠的、可重复的生物学结果。虽然由于波束时间的限制，这些研究还处于初步阶段，但研究结果总结如下：当x射线照射到Fe- k壳吸收边缘时，铁结合细胞的旁观者细胞死亡率低于边缘下能量x射线照射。当软x射线照射到S - k边缘吸收边缘时，被照射的细胞表现出比下边缘x射线照射的细胞更低的敏感性。对于旁观细胞的死亡，S - k边缘x射线的致死率高于下边缘x射线。我们将继续研究导致上述有趣生物现象的机制。