Research on biological effects in low dose region by using synchrotron X-ray microbeam irradiation system

同步辐射X射线微束辐照系统低剂量区生物效应研究

基本信息

项目摘要

Risk evaluation of low dose or low dose rate irradiation has been great concern for human societies utilizing modern technologies. In order to study biological effect of low level of radiation such as bystander effects, we have developed a new irradiation system with which radiation can be delivered to each cell individually using monochromatic synchrotron X-rays.Developed system is composed of three parts. The first part is to produce X-ray microbeam, either by a Kirkpatrick Baez (K-B) mirror system to focus the X-ray beam or a high-precision slit system to cut out the beam. The second is an epi-fluorescent microscope equipped with a precise motorized stage, on which the sample dish is fixed and irradiated with X-ray microbeam. The third is a fluorescence image analyzer (computer) with a sensitive CCD camera, which recognizes the target cells and their positions. It also controls irradiation of X-ray beam to the target cells, one by one, automatically. The system has been installed at BL-27B in the Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK) in Tsukuba, Japan. Minimum beam size of 5 μm square is now routinely exploited. Intensity of diffracted X-ray beam at the sample position is about 40 R/sec (ca. 10^4 photons/s in 10 μm square). Positioning accuracy of the targets, coordinates of which are automatically analyzed, are about 1 μm. Preliminary results of biological experiments are summarized as below.1. Corresponding to the size of the microbeam used, localization of γ-H2AX and other response proteins was observed in the nuclei of irradiated mammalian cells.2. Survival fractions of the microbeam-irradiated cells could successfully be obtained. By using the nucleus-averaged dose, the dose survival curve appears to be comparable with the case of wide beam experiments.
低剂量或低剂量率辐照的风险评估一直是利用现代技术的人类社会所关注的问题。为了研究低剂量辐射的生物效应,如旁观者效应,我们研制了一种新的辐射系统,利用单色同步辐射X射线将辐射分别照射到每个细胞上。第一部分是X射线微束的产生,通过柯克帕特里克-贝兹(K-B)反射镜系统聚焦X射线束或高精度狭缝系统切割X射线束。第二个是一个epi-fluorescent显微镜配备了一个精确的电动平台,其上的样品盘是固定的,用X射线微束照射。第三个是荧光图像分析仪(计算机),带有灵敏的CCD相机,可识别靶细胞及其位置。它还自动控制X射线束一个接一个地照射靶细胞。该系统已安装在BL-27 B的光子工厂,材料结构科学研究所,高能加速器研究组织(KEK)在日本筑波。现在常规采用5 μm见方的最小光束尺寸。样品位置处衍射X射线束的强度约为40 R/sec(约100 R/sec)。10 μm正方形中10^4光子/s)。自动分析目标坐标的定位精度约为1 μm。生物学实验的初步结果总结如下。相应于所用微束的大小,γ-H2 AX和其他反应蛋白在受照哺乳动物细胞核中的定位被观察到.可以成功地获得微波照射的细胞的存活分数。通过使用核平均剂量,剂量存活曲线似乎是可比的情况下,宽束实验。

项目成果

期刊论文数量(17)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Charged Particle and Photon Interactions with Matter
带电粒子和光子与物质的相互作用
  • DOI:
  • 发表时间:
    2010
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Kenji Takahashi;James Whishart
  • 通讯作者:
    James Whishart
Efficiencies of Double Strand Break Induction of DNA in Solution Induced by Photoabsorption at Phosphorus and
磷和光吸收诱导溶液中 DNA 双链断裂的效率
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    M.Maeda;K.Kobayashi;K.Hieda
  • 通讯作者:
    K.Hieda
放射光X線マイクロビーム照射装置を用いた研究の展望
同步加速器X射线微束辐照设备的研究前景
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    小林克己;宇佐美徳子
  • 通讯作者:
    宇佐美徳子
低線量放射線の生物影響研究のための放射光X線マイクロビーム細胞照射装置
研究低剂量辐射生物效应的同步辐射X射线微束细胞辐照装置
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    宇佐美徳子;小林克己
  • 通讯作者:
    小林克己
Study of Auger effect in DNA when bound to molecules containing platinum
DNA 与含铂分子结合时的俄歇效应研究
  • DOI:
  • 发表时间:
    2003
  • 期刊:
  • 影响因子:
    0
  • 作者:
    K.Kobyashi;N.Usami;I.Sasaki;H.Frohlich;C.Le Sech
  • 通讯作者:
    C.Le Sech
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KOBAYASHI Katsumi其他文献

機械接合におけるFRP母材の支圧耐力
机械连接中FRP基材的承载强度
  • DOI:
  • 发表时间:
    2012
  • 期刊:
  • 影响因子:
    0
  • 作者:
    INOUE Keiichi;KOBAYASHI Katsumi;ARAISHI Masafumi;川北拓未,松本幸大,山田聖志,中森啓太,小宮巌
  • 通讯作者:
    川北拓未,松本幸大,山田聖志,中森啓太,小宮巌
Structural characteristics of column with wing walls as energy absorption elements
以翼墙为吸能元件的柱结构特点
  • DOI:
  • 发表时间:
    2012
  • 期刊:
  • 影响因子:
    0
  • 作者:
    INOUE Keiichi;KOBAYASHI Katsumi;ARAISHI Masafumi
  • 通讯作者:
    ARAISHI Masafumi

KOBAYASHI Katsumi的其他文献

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{{ truncateString('KOBAYASHI Katsumi', 18)}}的其他基金

Rationalization of reinforcing arrangement design based on stress transfer mechanism of RC footing beams with single layer reinforcement for residential houses
基于单层钢筋混凝土基础梁应力传递机制的合理配筋设计
  • 批准号:
    22560558
  • 财政年份:
    2010
  • 资助金额:
    $ 33.78万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of a seismic retrofit technology for vulnerable buildings in developing countries
发展中国家脆弱建筑抗震改造技术的开发
  • 批准号:
    19560568
  • 财政年份:
    2007
  • 资助金额:
    $ 33.78万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Bystander effects induced with inner shell ionization followed by Auger effects
内壳电离引起的旁观者效应,随后是俄歇效应
  • 批准号:
    17201016
  • 财政年份:
    2005
  • 资助金额:
    $ 33.78万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Study of production mechanism of DNA clustered damage in aqueous solution
水溶液中DNA簇状损伤产生机制的研究
  • 批准号:
    10480137
  • 财政年份:
    1998
  • 资助金额:
    $ 33.78万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Ductility Improvement of Pre-cast Concrete Members by Intensive Shear reinforcement
通过强剪钢筋提高预制混凝土构件的延展性
  • 批准号:
    10650558
  • 财政年份:
    1998
  • 资助金额:
    $ 33.78万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Design criteria of FRP Reinforced Concrete Flexural Members
玻璃钢钢筋混凝土受弯构件设计规范
  • 批准号:
    07650656
  • 财政年份:
    1995
  • 资助金额:
    $ 33.78万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Mechanisms of molecular changes in amino acids due to inner shell ionization
内壳电离导致氨基酸分子变化的机制
  • 批准号:
    04453162
  • 财政年份:
    1992
  • 资助金额:
    $ 33.78万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Effect of the bond and dowel action of main bar on the shear behavior of RC members
主杆的粘结和销钉作用对钢筋混凝土构件抗剪性能的影响
  • 批准号:
    02650394
  • 财政年份:
    1990
  • 资助金额:
    $ 33.78万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

相似海外基金

Development of X-ray optics for medical imaging systems using synchrotron monochromatic X-ray
使用同步加速器单色 X 射线开发用于医学成像系统的 X 射线光学器件
  • 批准号:
    10670880
  • 财政年份:
    1998
  • 资助金额:
    $ 33.78万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
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