Construction of ultrahigh-density haplotyping devices using characteristics in nanospace

利用纳米空间特征构建超高密度单体分型装置

• 批准号: 17201032
• 负责人: BABA Yoshinobu
• 金额: $ 29.45万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17201032/
• 关键词: Micro-and Nanodevices; Genome; Nanobio; Fluid Simulation

We have developed ultrahigh-density haplotyping devices composed of ultra-fast genome analysis device, ultra-sensitive DNA detection device, and ultra-small reaction chamber system for genome analysis. In the first yean essential techniques in these component devices were developed. As a result, nanostructures inside microchannel were optimized for DNA sieving matrix and high sensitive detection using a novel DNA labeling method was achieved. Furthermore, it was demonstrated that the inkjet-type injection method did work as a DNA injectr. In the seccond year we investigated how to army microchannel in high density Since straight type microchannel is most suitable for making high-density microchannel array, combination of nanostructures and microchannel was optimized and ink-jet-type injector was mounted on the straight miceothannel We elucidated that this combination worked well as a DNA analysis device. In the last year final evaluation of the prototype device was performed. Combining all components that have been developed so fat highly integrated and high-density device was fabricated on a chip as a prototype device. Regarding to inkjet-type injector applied voltage, frequency, and wave pattern was optimized to reduce the distribution of volume of a single aliquot. After this process, we could inject 10 pL DNA sample into the straight type microchannel and electrophoretic DNA separation was successfully done. Although high-density haplotyping devices has same problems such as physical interference of peripheral equipment, we demonstrated highly integrated and high-density haplotyping devices could be realized using the above developed techniques.

我们开发了超高密度单体型分析设备，包括超快速基因组分析设备、超灵敏DNA检测设备和用于基因组分析的超小型反应室系统。在第一年，这些组件设备的基本技术得到了发展。因此，在微通道内的纳米结构进行了优化的DNA筛选矩阵和高灵敏度检测，使用一种新的DNA标记方法实现。此外，证明了喷墨型注射方法确实作为DNA注射剂起作用。第二年我们研究了如何高密度组装微通道，由于直型微通道最适合制作高密度微通道阵列，因此我们优化了纳米结构与微通道的组合，并在直型微通道上安装了喷墨式注射器，说明这种组合可以很好地用作DNA分析装置。去年，对原型器械进行了最终评价。结合所有已开发的元件，因此脂肪高度集成和高密度的设备被制作在一个芯片上作为原型设备。关于喷墨型注射器施加的电压，频率和波形进行了优化，以减少一个单一的等分试样的体积分布。经过这一过程，我们可以将10 pL的DNA样品注入到直型微通道中，并成功地进行了电泳DNA分离。虽然高密度单倍型分型装置具有相同的问题，例如外围设备的物理干扰，但我们证明了使用上述开发的技术可以实现高度集成和高密度单倍型分型装置。

项目成果

Microchip analysis of plant glucosinolates

• DOI: 10.1002/elps.200700635
• 发表时间: 2008-06-01
• 期刊: ELECTROPHORESIS
• 影响因子: 2.9
• 作者: Fouad, Maged;Jabasini, Mohammad;Baba, Yoshinobu
• 通讯作者: Baba, Yoshinobu

DNA microarray analysis of type 2 diabetes-related genes co-regulated between white blood cells and livers of diabetic Otsuka long-evans Tokushima fatty (OLETF) rats

• DOI: 10.1248/bpb.30.763
• 发表时间: 2007-04-01
• 期刊: BIOLOGICAL & PHARMACEUTICAL BULLETIN
• 影响因子: 2
• 作者: Hayashi, Yasuhiro;Iida, Shinya;Harashima, Hideyoshi
• 通讯作者: Harashima, Hideyoshi

Electrophoretic behavior of plasmid DNA in the presence of various intercalating dyes

不同嵌入染料存在下质粒 DNA 的电泳行为

• 发表时间: 2006
• 期刊: J. Chromatogr. A 1118
• 作者: F. Dang;et. al.
• 通讯作者: et. al.

Effect of polymer matrix and glycerol on rapid single-strand conformation polymorphism analysis by capillary and microchip electrophoresis for detection of mutations in K-ras gene

聚合物基质和甘油对毛细管和微芯片电泳快速单链构象多态性分析检测 K-ras 基因突变的影响

• 发表时间: 2005
• 期刊: Electrophoresis 26
• 作者: Y. Endo;et. al.
• 通讯作者: et. al.

Rapid Single-Strand Conformation Polymorphism Analysis by Capillary and Microchip Electrophoresis for Detecting Mutations in K-ras Gene

通过毛细管和微芯片电泳快速单链构象多态性分析检测 K-ras 基因突变

• 发表时间: 2005
• 期刊: Electrophoresis 26
• 作者: Y.Endo;et al.
• 通讯作者: et al.