System biologic approach to lipid traffic and conversion in eukaryotic microorganisms and its application

真核微生物脂质运输和转化的系统生物学方法及其应用

基本信息

  • 批准号:
    17208008
  • 负责人:
  • 金额:
    $ 31.37万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
  • 财政年份:
    2005
  • 资助国家:
    日本
  • 起止时间:
    2005 至 2007
  • 项目状态:
    已结题

项目摘要

This project was comprised of two parts : One is the analysis on the uptake and acyl-chain remodeling of phospholipids with short fatty acid residues by yeast Saccharomyces cerevisiae. In previous research, we showed that phosphatidylethanolamine (PE) and phosphatidylcholine (PC) with 8-to 12-carbon-fatty acid residues were incorporated and utilized by S. cerevisiae mutants defective in the synthesis of these phospholipids. In this project, we proved that these soluble phospholipids were. transported into the cells and their acyl chains are actually replaced with those of normal length by tracing the change of stable isotope-labeled PE and PC with decanoic acids. Thus the remodeling of phospholipids in their fatty acid residues is a common property of eukaryotic cells.The other part is the finding of a negative transcription factor, Yas3, for alkane-induced gene expression in Yarrowia lipolytica. In our previous research, we reported two cooperative positive regulatory factors, Yaslp and Yas2p, with bHLH motifs. We found that Yas3p changed its intracellular localization. When the lipolytica cells were grown in the presence of alkane, Yas3p-GFP was found in ER, but in the absence of alkane, it was found in nucleus, where it likely represses the function of Yas1p and Yas2p by binding to Yas2p. We also found Yas3p specifically bound to phosphatidic acid and PA phosphatase mutation increased expression of the alkane-inducible ALK1 gene, suggesting the change in the amount of PA is important in the function of Yas3p. We also investigated expression of 12 ALK genes by RT-PCR and found not all ALK genes are responsive to alkane.The latter part of our project resulted in a discovery of a very strong inducible promoter in Y. lipolytica and a mutant strain that is suitable for the expression of cytochrome P450 genes.
本课题主要包括两个部分:一是酵母菌对短脂肪酸残基磷脂的摄取和酰基链重塑的研究。在以往的研究中,我们发现,磷脂酰乙醇胺(PE)和磷脂酰胆碱(PC)的8-至12-碳脂肪酸残基的整合和利用的S。酿酒酵母突变体在这些磷脂的合成中有缺陷。在这个项目中,我们证明了这些可溶性磷脂。通过用癸酸追踪稳定同位素标记的PE和PC的变化,它们的酰基链实际上被正常长度的酰基链取代。因此磷脂在其脂肪酸残基中的重构是真核细胞的共同特性。另一部分是在解脂耶氏酵母中发现了烷烃诱导基因表达的负转录因子Yas 3。在我们以前的研究中,我们报道了两个具有bHLH基序的协同正调控因子Yaslp和Yas 2 p。我们发现Yas 3 p改变了其细胞内定位。当解脂细胞在有烷烃存在的情况下生长时,在ER中发现了Yas 3 p-GFP,但在没有烷烃存在的情况下,在细胞核中发现了Yas 3 p-GFP,它可能通过与Yas 2 p结合来抑制Yas 1 p和Yas 2 p的功能。我们还发现Yas 3 p特异性结合磷脂酸和PA磷酸酶突变增加了烷烃诱导的ALK 1基因的表达,表明PA量的变化在Yas 3 p的功能中是重要的。我们还通过RT-PCR检测了12个ALK基因的表达,发现并非所有的ALK基因都对烷烃有反应。和适于表达细胞色素P450基因的突变菌株。

项目成果

期刊论文数量(39)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Crystallization and preliminary X-ray analsis of CTP : phosphoethanolamine cytidylyltransferase(ECT)from Saccharomyces cerevisiae.
CTP 的结晶和初步 X 射线分析:来自酿酒酵母的磷酸乙醇胺胞苷酰转移酶(ECT)。
  • DOI:
  • 发表时间:
    2006
  • 期刊:
  • 影响因子:
    0
  • 作者:
    J.Ohtsuka;K.Nagata 他5名
  • 通讯作者:
    K.Nagata 他5名
Crystallization and preliminary X-ray analsis of CTP : phosphoethanolamine cytidylyltransferase(ECT)from Saccharomyces cerevisiae
酿酒酵母磷酸乙醇胺胞苷酰转移酶 (ECT) 的结晶和初步 X 射线分析
  • DOI:
  • 发表时间:
    2006
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Jun Ohtsuka;Koji Nagata;Woo Ceol Lee;Yusuke Ono;Ryouichi Fukuda;Akinori Ohta;Masaru Tanokura
  • 通讯作者:
    Masaru Tanokura
The Bax Inhibitor-1 needs a functional electron transport chain for cell death suppression
Bax Inhibitor-1 需要功能性电子传递链来抑制细胞死亡
  • DOI:
  • 发表时间:
    2007
  • 期刊:
  • 影响因子:
    0
  • 作者:
    R. Oshima;K. Yoshinaga;他5名
  • 通讯作者:
    他5名
Incorporation and Remodeling of Extracellular Phosphatidylcholine in Saccharomycescerevisiae
酿酒酵母细胞外磷脂酰胆碱的掺入和重塑
  • DOI:
  • 发表时间:
    2007
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Fukuda R.;Tanaka K.;Eguchi H.;Ono Y.;Nagasawa S.;Nakatani Y.;Watanabe H.;Nakanishi H.;Taguchi R.;and Ohta A.
  • 通讯作者:
    and Ohta A.
Yas3p, an Opi1 Family Transcription Factor, Regulates Cytochrome P450 Expression in Response to n-Alkanes in Yarrowia lipolytica
  • DOI:
    10.1074/jbc.m806864200
  • 发表时间:
    2009-03-13
  • 期刊:
  • 影响因子:
    4.8
  • 作者:
    Hirakawa, Kiyoshi;Kobayashi, Satoshi;Ohta, Akinori
  • 通讯作者:
    Ohta, Akinori
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OHTA Akinori其他文献

OHTA Akinori的其他文献

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{{ truncateString('OHTA Akinori', 18)}}的其他基金

Study on lipid metabolism in the industrial yeast Yarrowia lipolytica
工业酵母解脂耶氏酵母脂质代谢研究
  • 批准号:
    24380045
  • 财政年份:
    2012
  • 资助金额:
    $ 31.37万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Study on molecular mechanisms of uptake, conversion, and excretion of lipids in yeast.
酵母脂质摄取、转化和排泄的分子机制研究。
  • 批准号:
    14206010
  • 财政年份:
    2002
  • 资助金额:
    $ 31.37万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Improvement of long chain dicarboxylic acid production by fortifying omega-oxidation, excretion of products and lipase production in yeast
通过强化酵母中的欧米伽氧化、产物排泄和脂肪酶生产来改善长链二羧酸的生产
  • 批准号:
    12556011
  • 财政年份:
    2000
  • 资助金额:
    $ 31.37万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Mechanism-Based Biodegradability Prediction of Dehalogenase
基于机制的脱卤酶生物降解性预测
  • 批准号:
    10044197
  • 财政年份:
    1998
  • 资助金额:
    $ 31.37万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
Analysis on the mechanism of ER membrane proliferation upon ER protein overproduction in yeast
酵母内质网蛋白过量产生内质网膜增殖机制分析
  • 批准号:
    10460034
  • 财政年份:
    1998
  • 资助金额:
    $ 31.37万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Construction of terminal oxidation systems for hydrocabon chain A use of a variety of yeast cytochrome P450 genes
多种酵母细胞色素P450基因的烃链末端氧化系统的构建
  • 批准号:
    10556015
  • 财政年份:
    1998
  • 资助金额:
    $ 31.37万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Breeding of a flocculent, highly alcohol-producing yeast
絮状高产酒精酵母的育种
  • 批准号:
    08556012
  • 财政年份:
    1996
  • 资助金额:
    $ 31.37万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Study on the development and differentiation of endoplasmic reticulum of yeast from the view point of lipid synthesis.
从脂质合成的角度研究酵母内质网的发育和分化。
  • 批准号:
    07456045
  • 财政年份:
    1995
  • 资助金额:
    $ 31.37万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Genetic Engineering of Long Chain Dicarboxylic Acid Production in Yeast
酵母生产长链二元酸的基因工程
  • 批准号:
    06556013
  • 财政年份:
    1994
  • 资助金额:
    $ 31.37万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research (B)
Role of Amino-phospholipid Synthesis in Biogenesis of Endoplasmic Reticulum of Yeast
氨基磷脂合成在酵母内质网生物合成中的作用
  • 批准号:
    04660080
  • 财政年份:
    1992
  • 资助金额:
    $ 31.37万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

相似国自然基金

低pH胁迫下Yarrowia lipolytica代谢机制解析及响应型启动子的挖掘与应用
  • 批准号:
  • 批准年份:
    2022
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  • 项目类别:
    青年科学基金项目

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Sustainable Bioproduction of natural dyes in the yeast Yarrowia lipolytica
解脂耶氏酵母天然染料的可持续生物生产
  • 批准号:
    2735012
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卓越研究:PAH1 介导的模型产油酵母解脂耶氏酵母中脂质合成的调节
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    2100980
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    2021
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    Standard Grant
Using microbial co-cultures involving Yarrowia lipolytica to produce sesquiterpenoids
使用涉及解脂耶氏酵母的微生物共培养来生产倍半萜类化合物
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    2505903
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用解脂耶氏酵母设计基于酵母的新型食品:结合营养、合成生物学和可持续性的综合方法
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    2504639
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    2018
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Yarrowia lipolytica as a viable host platform for heterologous production of tanshinones
解脂耶氏酵母作为异源生产丹参酮的可行宿主平台
  • 批准号:
    405772767
  • 财政年份:
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Regulation of phosphatidic acid phosphatase during lipogenesis in the oleaginous yeast Yarrowia lipolytica Phosphatidic acid phosphatase in Yarrowia lipolytica
产油酵母解脂耶氏酵母脂肪生成过程中磷脂酸磷酸酶的调节 解脂耶氏酵母中的磷脂酸磷酸酶
  • 批准号:
    9377751
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Engineering the yeasts Saccharomyces cerevisiae and Yarrowia lipolytica for production of fatty alcohols.
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    $ 31.37万
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    Postdoctoral Fellowships
Engineering the yeasts Saccharomyces cerevisiae and Yarrowia lipolytica for production of fatty alcohols.
对酿酒酵母和解脂耶氏酵母进行工程改造以生产脂肪醇。
  • 批准号:
    471688-2015
  • 财政年份:
    2015
  • 资助金额:
    $ 31.37万
  • 项目类别:
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EAGER: Enabling Direct Microbial Biotransformation of Methane and Derived Methanol to Valuable Biochemicals and Biofuels by Yarrowia Lipolytica
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  • 批准号:
    1360867
  • 财政年份:
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Engineering the yeasts Saccharomyces cerevisiae and Yarrowia lipolytica for production of fatty alcohols.
对酿酒酵母和解脂耶氏酵母进行工程改造以生产脂肪醇。
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    471688-2015
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