Improvement of long chain dicarboxylic acid production by fortifying omega-oxidation, excretion of products and lipase production in yeast

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Result of this research is constituted of following two parts.From comparison of wild type and the long chain dicarboxylic acid (DCA)-producing mutant of Candida maltosa, we found that ALK5 gene that is active in ω-oxidation of fatty acids was highly produced in the mutant, which is presumed to be important for DCA production. Improvement of ALK5 expression by alteration of promoter structure should be useful try in future.We also found that a gne encoding one of ABC transporters was strongly induced at the time of DCA production in the mutant.. The identified gene was a homolog of CDR1 of Candida albicance, which confers cycloheximide resistance to this pathogenic yeast. Further investigation is necessary for full elucidation of its function in DCA production in C. maltosa.