Breeding of a flocculent, highly alcohol-producing yeast

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The control of yeast aggregation is desirable for alcohol fermentation industry, because flocculent yeast by aggregation can be easily removed from the fermenter and reused for next successive fermentation. Aiming development of such a technique, we introduced FL01, a Saccharomyces cerevisiae gene that causes flocculation, into an industrial alcohol-fermenting S.Cerevisiae strain 396. In consideration with inevitable escape of yeast cells form large scale of fermentation and the purpose of the pruduct alcohol for food and pharmaceuticals, it is desirable that the fermenting yeast does not contain heterologous genes. We, therefore, inserted the homologous ADH1^P-FL01 gene into URA3 of the strain 396. The resultant recombinat was flocculent and comparable in capability of alcohol production with the parental industrial S.cerevisiae strain 396.