Breeding of a flocculent, highly alcohol-producing yeast

絮状高产酒精酵母的育种

• 批准号: 08556012
• 负责人: OHTA Akinori
• 金额: $ 3.39万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08556012/
• 关键词: Saccharomyces cerevisiae; yeast; alcohol fermentation; self cloning; flocculation; FL01; yeast breeding; targeted gene manipulation; セルフクローニング; Saccharomyos cerevisiae; 凝集性; アルコール生産; 酵母

The control of yeast aggregation is desirable for alcohol fermentation industry, because flocculent yeast by aggregation can be easily removed from the fermenter and reused for next successive fermentation. Aiming development of such a technique, we introduced FL01, a Saccharomyces cerevisiae gene that causes flocculation, into an industrial alcohol-fermenting S.Cerevisiae strain 396. In consideration with inevitable escape of yeast cells form large scale of fermentation and the purpose of the pruduct alcohol for food and pharmaceuticals, it is desirable that the fermenting yeast does not contain heterologous genes. We, therefore, inserted the homologous ADH1^P-FL01 gene into URA3 of the strain 396. The resultant recombinat was flocculent and comparable in capability of alcohol production with the parental industrial S.cerevisiae strain 396.

控制酵母的聚集是酒精发酵工业所希望的，因为通过聚集的絮状酵母可以容易地从发酵罐中去除并重新用于下一次连续的发酵。为了开发这种技术，我们将导致絮凝的酿酒酵母基因FL 01导入工业酒精发酵酿酒酵母菌株396中。考虑到酵母细胞不可避免地从大规模发酵中逃逸以及产品酒精用于食品和药物的目的，希望发酵酵母不含异源基因。因此，我们将同源ADH 1-P-FL 01基因插入到菌株396的URA 3中。该重组子具有絮凝性，产酒精能力与亲本工业酿酒酵母396相当。