Development of diagnostic tests for infectious diseases of mice and rats by microsphere fluorescent immunoassay

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Microsphere fluorescent immunoassay (MFI) allows simultaneous detection of antibodies to multiple rodent pathogens using a small volume of sera. In the present study, we examined the specificity and sensitivity of MFI in serologic diagnosis for infections on mice and rats. Antibodies to major 9 pathogens in mice and rats were measured by MFI using recombinant and inactivated antigens. MFI succeeded in multiplex detection of antibodies to the above-mentioned pathogens in a single reaction well without decreasing its specificity and sensitivity. MFI is highly suitable for serodiagnosis in laboratory mice and rats.