Development of diagnostic tests for infectious diseases of mice and rats by microsphere fluorescent immunoassay

微球荧光免疫分析法诊断小鼠和大鼠传染病的研究进展

• 批准号: 19200033
• 负责人: YAGAMI Ken-ichi
• 金额: $ 31.03万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-19200033/
• 关键词: 実験動物; 微生物検査; 血清診断; マイクロビーズ法; マウス; ラット; 微生物モニタリング; 微量検査法; 組換え抗原

Microsphere fluorescent immunoassay (MFI) allows simultaneous detection of antibodies to multiple rodent pathogens using a small volume of sera. In the present study, we examined the specificity and sensitivity of MFI in serologic diagnosis for infections on mice and rats. Antibodies to major 9 pathogens in mice and rats were measured by MFI using recombinant and inactivated antigens. MFI succeeded in multiplex detection of antibodies to the above-mentioned pathogens in a single reaction well without decreasing its specificity and sensitivity. MFI is highly suitable for serodiagnosis in laboratory mice and rats.

微球荧光免疫测定（MFI）允许使用少量血清同时检测多种啮齿动物病原体的抗体。在本研究中，我们检测了MFI在小鼠和大鼠感染的血清学诊断中的特异性和敏感性。采用重组抗原和灭活抗原，MFI检测小鼠和大鼠对9种主要病原体的抗体。MFI在不降低特异性和敏感性的情况下，成功地在一次反应中对上述病原体的抗体进行了多重检测。MFI对实验室小鼠和大鼠的血清诊断具有很高的适用性。

项目成果

First Trial in the Developmental Phase of the "Performance Evaluation Program" Based on the ICLAS Animal Quality Network Program: Self-Assessment of Microbiological Monitoring Methods Using Test Samples Supplied by ICLAS

• DOI: 10.1538/expanim.58.47
• 发表时间: 2009-01-01
• 期刊: EXPERIMENTAL ANIMALS
• 影响因子: 2.4
• 作者: Goto, Kazuo;Hayashimoto, Nobuhito;Kagiyama, Naoko
• 通讯作者: Kagiyama, Naoko

腎症候性出血熱(HFRS)とハンタウイルス肺症候群(HPS)-げっ歯類媒介性の人獣共通感染症-

肾综合征出血热 (HFRS) 和汉坦病毒肺综合征 (HPS) - 啮齿动物传播的人畜共患疾病 -

• 发表时间: 2007
• 作者: 和田 圭司;他;有川二郎
• 通讯作者: 有川二郎

マウス肝炎ウイルスの組換え抗原

小鼠肝炎病毒重组抗原

• 发表时间: 2010

LCMV infection in a wild-derived inbred strain undetected by dirty bedding sentinel health monitoring and revealed after embryo transfer

肮脏的垫料哨点健康监测未检测到野生近交系中的 LCMV 感染，并在胚胎移植后发现

• 发表时间: 2007
• 期刊: Comparative Medicine 57
• 作者: Ike;F.;Bourgade;F.;Ohsawa;K.;Sato;H.;Morikawa;S.;Takimoto;K.;J. Jaubert;Berard;M.;Nakata;H.;Hiraiwa;N.;Takakura;A.;Itoh;T.;Obata;Y.;Yoshiki;A. and X. Montagutelli
• 通讯作者: A. and X. Montagutelli

Comparative analysis of Pasteurella pneumotropica isolates from laboratory mice and rats

实验室小鼠和大鼠分离的嗜肺巴氏杆菌的比较分析

• 发表时间: 2009
• 期刊: Antonie Van Leewenhoek 95
• 作者: Sasaki H.;et al.
• 通讯作者: et al.