Field trial of transgenic poplars for industrial use

以杨树为试材，研究了曲霉木葡聚糖酶cDNA（AaXEG2）在35S启动子下的转基因表达。这一水平略有下降，杨树生长在肥沃的土壤中四年。木葡聚糖含量大大降低，在转基因株系的组织壁，虽然木葡聚糖的降解水平略低于那些生长在肥沃的土壤中的杨树比生长在非肥沃的土壤。与野生型植物相比，叶子表现出更小的表面积和更圆的齿，就好像它们是在孵育室中生长并随后被温室化的太阳叶品种。通过蛋白质组学分析，大量的蛋白质在转基因植物的叶片中显著下调。无论是活茎还是干茎，其弹性模量和断裂模量均显著低于野生型。

The transgenic expression of Aspergillus xyloglucanase cDNA(AaXEG2) with 35S promoter in the leaves of open-field grown poplars was studied. This level was slightly decreased in the poplars grown in fertile soil for four years. Xyloglucan content was greatly decreased in the walls of the tissues in the transgenic lines, although the degradation levels of xyloglucan were slightly lower for the poplars grown in fertile soil than for those grown in non-fertile soil. The leaves exhibited a smaller surface area with more rounded teeth than did those of the wild type plants, as if they were of the sun leaf variety that was grown in the incubation room and subsequently greenhoused. Large numbers of proteins were markedly downregulated in the leaves of the transgenic plants via proteomic analysis. Significant reduction occurred not only on the moduli of elasticity and rupture in both their live stems and dried trunks compared with those in the wild type.