Field trial of transgenic poplars for industrial use

工业用转基因杨树田间试验

• 批准号: 19208016
• 负责人: HAYASHI Takahisa
• 金额: $ 30.28万
• 依托单位: Tokyo University of Agriculture (2009-2010)Kyoto University (2007-2008)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-19208016/
• 关键词: 組織構造; 材形成; キシログルカン; ポプラ; 野外試験; キシログルカナーゼ; 二酸化炭素排出削減; バイオテクノロジー; 森林工学; 植物; 一酸化炭素排出削減; バイオマス; バイオテクノロジーー

The transgenic expression of Aspergillus xyloglucanase cDNA(AaXEG2) with 35S promoter in the leaves of open-field grown poplars was studied. This level was slightly decreased in the poplars grown in fertile soil for four years. Xyloglucan content was greatly decreased in the walls of the tissues in the transgenic lines, although the degradation levels of xyloglucan were slightly lower for the poplars grown in fertile soil than for those grown in non-fertile soil. The leaves exhibited a smaller surface area with more rounded teeth than did those of the wild type plants, as if they were of the sun leaf variety that was grown in the incubation room and subsequently greenhoused. Large numbers of proteins were markedly downregulated in the leaves of the transgenic plants via proteomic analysis. Significant reduction occurred not only on the moduli of elasticity and rupture in both their live stems and dried trunks compared with those in the wild type.

以杨树为试材，研究了曲霉木葡聚糖酶cDNA（AaXEG2）在35S启动子下的转基因表达。这一水平略有下降，杨树生长在肥沃的土壤中四年。木葡聚糖含量大大降低，在转基因株系的组织壁，虽然木葡聚糖的降解水平略低于那些生长在肥沃的土壤中的杨树比生长在非肥沃的土壤。与野生型植物相比，叶子表现出更小的表面积和更圆的齿，就好像它们是在孵育室中生长并随后被温室化的太阳叶品种。通过蛋白质组学分析，大量的蛋白质在转基因植物的叶片中显著下调。无论是活茎还是干茎，其弹性模量和断裂模量均显著低于野生型。

项目成果

Overexpression of DnaK from a halotolerant cyanobacterium Aphanothece halophytica enhances growth rate as well as abiotic stress tolerance of poplar plants

耐盐蓝藻 Aphanothece halophytica 过表达 DnaK 可提高杨树的生长速度和非生物胁迫耐受性

• 发表时间: 2008
• 期刊: Plant Growth Reg
• 作者: T Takabe T;A Uchida A;F Shinagawa;Y Terada;H Kajita;Y Tanaka;T Takabe;T Hayashi;T Kawai;T Takabe
• 通讯作者: T Takabe

キシログルカナーゼを過剰発現させた組換えポプラの野外での成長と根萌芽の発生について

过表达木葡聚糖酶的重组杨的田间生长和根系萌发

• 发表时间: 2011
• 作者: Won NI;Kawamura T;Takami H;Watanabe Y;谷口亨・栗田学・小長谷賢一・石井克明・舟橋史晃・太田誠一・馬場啓一・加来友美・海田るみ・林隆久
• 通讯作者: 谷口亨・栗田学・小長谷賢一・石井克明・舟橋史晃・太田誠一・馬場啓一・加来友美・海田るみ・林隆久

Overexpression of xyloglucanase(AaXEG2) accelerates heteroblastic development in mangium leaves

木葡聚糖酶（AaXEG2）的过度表达加速马曱叶异母细胞发育

• 发表时间: 2011
• 期刊: J Wood Sci
• 影响因子: 2.9
• 作者: Hartati S;Sudarmonowati E;Kaku T;Ikegaya H;Baba K;Kaida R;Hayashi T
• 通讯作者: Hayashi T

Improvement of fermentable sugar yields of mangium through transgenic overexpression of xyloglucan

通过木葡聚糖转基因过表达提高马占的可发酵糖产量

• 发表时间: 2011
• 期刊: Journal Wood Science
• 作者: Tomomi Kaku;Kei'ichi Baba;Toru Taniguchi;Takahisa Hayashi
• 通讯作者: Takahisa Hayashi

Plasticity of xyloglucan composition in bean (Phaseolus vulgaris)-cultured cells during habituation and dehabituation to lethal concentrations of dichlobenil.

• DOI: 10.1093/mp/ssq011
• 发表时间: 2010-05
• 期刊: Molecular plant
• 影响因子: 27.5
• 作者: Ana Alonso-Simón;Lutz Neumetzler;P. García-Angulo;A. Encina;J. Acebes;J. Álvarez;Takahisa Hayashi