Pattern-recognition receptors in dendritic cells evoke cellular immunity in response to viral RNA patterns

树突状细胞中的模式识别受体根据病毒 RNA 模式激发细胞免疫

• 批准号: 19209015
• 负责人: SEYA Tsukasa
• 金额: $ 31.12万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-19209015/
• 关键词: 自然免疫; TLR; TICAM-1; IPS-1; 樹状細胞; NK細胞; RNAパターン認識; ワクチン; Toll-like receptor; TICAM-1経路; Toll-like-receptor; Myd88経路; polyI:C; TICAM-1(TRIF); NK activation; Dendritic cell; RNA adjuvant; antitumor therapy; interferon (IFN); adoptive trans

How host cells respond to viral dsRNA is analyzed in this study. Dendritic cells (DC) stimulated with polyI : C (an dsRNA analog) render the DC competent for NK activation. Since cell-cell contact was essential for this NK activation, the molecule expressed on DC to drive NK activation was identified from genes up-regulated by polyI : C stimulation. Ultimately, we identified a membrane protein responsible for NK induction and named it INAM. INAM is a tetraspanin-like molecule inducing NK tumoricidal activity and in vivo tumor regression.

本研究分析了宿主细胞如何响应病毒 dsRNA。用 PolyI : C（一种 dsRNA 类似物）刺激的树突状细胞 (DC) 使 DC 能够激活 NK。由于细胞间接触对于 NK 激活至关重要，因此从受 PolyI:C 刺激上调的基因中鉴定出 DC 上表达的驱动 NK 激活的分子。最终，我们鉴定了一种负责 NK 诱导的膜蛋白，并将其命名为 INAM。 INAM 是一种四跨膜蛋白样分子，可诱导 NK 杀肿瘤活性和体内肿瘤消退。

项目成果

Functional evolution of the TICAM-1 (TRIF) pathway. for extrins is RNA sensing. (review)

TICAM-1 (TRIF) 通路的功能进化。

• 发表时间: 2009
• 期刊: Immunol. Rev. 227
• 作者: Seya;T.;et.al.
• 通讯作者: et.al.

Homo-oligomerization is essential for Toll/IL-1 receptor domain containing adaptor molecule-1-mediated NF-kappaB and interferon regulatory factor-3 activation

同源寡聚对于含有接头分子 1 介导的 Toll/IL-1 受体结构域和干扰素调节因子 3 的激活至关重要

• 发表时间: 2008
• 期刊: J. Biol. Chem. 283
• 作者: Funami K.;M. Sasai;H. Oshiumi;T. Seya;M. Matsumoto
• 通讯作者: M. Matsumoto

Recombinant IL-12/IL-18 antitumor therapy in a guinea pig hepatoma cell implanted model

重组IL-12/IL-18抗肿瘤治疗豚鼠肝癌细胞移植模型

• 发表时间: 2007
• 期刊: Cancer Sci. 98
• 作者: Shiratori;I.;Y. Suzuki;H. Oshiumi;N. A. Begum;T. Ebihara;M. Matsumoto;K. Hazeki;K. Kodama;Y. Kashiwazaki;T. Seya.
• 通讯作者: T. Seya.

Obstructing shedding of the immune stimulatory MHC class I chain-related gene B prevents tumor formation

阻碍免疫刺激 MHC I 类链相关基因 B 的脱落可预防肿瘤形成

• 发表时间: 2009
• 期刊: Clin.Cancer Res. 15
• 作者: Wu;J.D.;C.L.Atteridge;X.J.Wang;T.Seya;S.R.Plymate
• 通讯作者: S.R.Plymate

Teleost TLR22 recognizes RNA duplex to induce IFN and protect cells from birnaviruses

• DOI: 10.4049/jimmunol.181.5.3474
• 发表时间: 2008-09-01
• 期刊: JOURNAL OF IMMUNOLOGY
• 影响因子: 4.4
• 作者: Matsuo, Aya;Oshiumi, Hiroyuki;Seya, Tsukasa
• 通讯作者: Seya, Tsukasa