Identification and automated counting of egg and larvae of zooplankton by molecular probes and its application to life history analysis

浮游动物卵和幼虫的分子探针识别、自动计数及其在生活史分析中的应用

• 批准号: 20241003
• 负责人: TSUDA Atsushi
• 金额: $ 31.37万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20241003/
• 关键词: 分子マーカー; 動物プランクトン; 自動計測; 生物海洋; 環境計測; 初期生活史

The coal of this study was to developments of species identification with molecular probes and automated counting system. We have developed species identification methods by real-time PCR for Neocalanus copepods, although we have failed to develop a automated counting system with flow-cytometer by technical problems. Using this method, we revealed early life histories of three species of Neocalanus copepods. Especially, one species of Neocalanus newly showed dormancy during their nauplius stages.

本研究的目的是发展分子探针和自动计数系统在物种鉴定中的应用。我们已经建立了新哲水蚤桡足类的实时荧光定量PCR鉴定方法，但由于技术问题，未能建立流式细胞仪自动计数系统。利用这种方法，我们揭示了三种新哲水蚤桡足类的早期生活史。特别是新哲水蚤属的一个种在无节幼体阶段新表现出休眠。

项目成果

Meso- and microzooplankton responses to an in situ iron fertilization experiment (SEEDS II) in the northwest subarctic Pacific

• DOI: 10.1016/j.dsr2.2009.06.004
• 发表时间: 2009-12
• 期刊: Deep-sea Research Part Ii-topical Studies in Oceanography
• 影响因子: 3
• 作者: A. Tsuda;H. Saito;R. Machida;S. Shimode

浮遊性カイアシ類の両極および反赤道分布について

浮游桡足类的双极和反​​赤道分布

• 期刊: 化石 (印刷中)
• 作者: 大塚攻;谷村篤;西田周平;町田龍二
• 通讯作者: 町田龍二

生物学辞典

生物学词典

• 作者: 浜田盛久;川本竜彦;高橋栄一;藤井敏嗣;江崎洋一
• 通讯作者: 江崎洋一

Response of lower trophic organisms to nutrients input and effects on carbon budget: a mesocosm experiment.

低营养生物对营养物质输入的响应及其对碳预算的影响：中生态实验。

• 发表时间: 2010
• 期刊: Plankton and Benthos Research
• 影响因子: 0.6
• 作者: Tsuda;A.;K. Fukami;H. Kiyosawa;K.Suzuki;S. Takeda;J. Nishioka;M. Takahashi;K.W. Johnson;C. S. Wong
• 通讯作者: C. S. Wong

Distribution and life history of two planktonic copepods, Rhincalanus nasutus and Rhincalanus rostrifrons, in the northwestern Pacific Ocean.

西北太平洋两种浮游桡足类动物Rhincalanus nasutus 和Rhincalanus rostrifrons 的分布和生活史。

• 发表时间: 2012
• 期刊: Deep Sea Research I
• 作者: Shimode;S;K. Takahashi;Y. Shimizu;T. Nonomura and A. Tsuda
• 通讯作者: T. Nonomura and A. Tsuda