ユビキタス海洋動物プランクトン、ワカレオタマボヤの集団ゲノム科学

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In FY2021, I contributed to two studies on genetic variation in O. dioica. The first reports that the species O. dioica may in fact be a species complex representing multiple lineages. The second demonstrates a considerable degree of genetic rearrangement in these species, which is unique among chordates and perhaps all metazoa.We began follow-up studies to examine the effects of this rearrangement on gene expression, achieving two important milestones in FY2021. In FY2020, we established the ATACseq method. In FY2021 we completed sampling and sequencing for RNAseq and ATACseq in two O. dioica populations, across developmental time points. We also established the hybridization chain reaction in O. dioica to accurately observe the spatiotemporal patterns of expression in real time.