Analysis of anticancer drug-resistant ovarian cancer cells under hypoxic tumor microenvironment

与紫杉醇敏感细胞相比，紫杉醇耐药卵巢癌细胞显示Akt的活化和Akt2的表达水平显著升高。HIF-2α在紫杉醇耐药细胞中的表达也高于紫杉醇敏感细胞。接下来，我们通过选择性抑制Akt2来检测Akt2在紫杉醇耐药体内肿瘤生长中的意义。靶向Akt2的siRNA抑制了裸鼠卵巢肿瘤的生长。这些结果表明，针对Akt2或HIF-2α的分子靶向治疗对紫杉醇耐药卵巢癌是有用的。

Paclitaxel-resistant ovarian cancer cells showed the activation of Akt and significantly higher levels of Akt2 expression compared with paclitaxel-sensitive cells. An HIF-2α expression was also higher in paclitaxel-resistant cells than in paclitaxel-sensitive cells.We next examined the significance of Akt2 in paclitaxel-resistant in vivo tumor growth by selectively inhibiting Akt2. An siRNA targeting Akt2 inhibited ovarian tumor growth in nude mice. These results indicated molecular target treatment against either Akt2 or HIF-2α would be useful for the paclitaxel-resistant ovarian cancer.