Regeneration of invertebral disc by iPS cells and challenge for in situ iPS cell bank.

iPS 细胞对椎间盘的再生以及对原位 iPS 细胞库的挑战。

• 批准号: 21890140
• 负责人: KAKUTANI Kenichiro
• 金额: $ 1.66万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21890140/
• 关键词: iPS細胞; 椎間板再生; 生体内バンク; 間葉系幹細胞; 椎間板細胞

The stable method to differentiate intervertebral disc (IVD) cell from iPS cells has not been established yet. Here we employed the co-culture method with iPS cells and IVD cells to differentiate iPS cells. At first, we directly co-cultured iPS cells with IVD cells. However iPS cells differentiated fibroblast like cells, not have the characteristics of IVD cells. Next we induced embryoid body from iPS cells, in addition embryoid body cells were treated with retinoic acid for seven days. After treatment, mesenchymal stem like cells were differentiated. Furthermore these differentiated cells were co-cultured with osteoblast inducer reagent for three weeks. We have confirmed the potential of mesenchymal stem like cells to differentiate the osteoblast cell by Alizarin red staining.

目前还没有建立稳定的区分IVD细胞和iPS细胞的方法。本实验采用iPS细胞与IVD细胞共培养的方法分化iPS细胞。首先，我们直接将iPS细胞与IVD细胞共培养。但iPS细胞分化为成纤维细胞样细胞，不具备IVD细胞的特征。接下来我们从iPS细胞中诱导出类胚体，并用维甲酸处理类胚体细胞7天。治疗后，间充质干细胞样细胞分化。将分化后的细胞与成骨细胞诱导剂共培养3周。茜素红染色证实了间充质干细胞向成骨细胞分化的潜能。

项目成果

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