Regeneration ofintervertebral disc using iPS cell and application of in vivo iPS cell bank.

我们采用iPS细胞和IVD细胞共培养的方法来分化iPS细胞。首先，我们直接将iPS细胞与IVD细胞共培养。我们从iPS细胞中诱导出类胚体，并用维甲酸处理7天。处理后，向间充质干细胞样细胞分化，并与成骨细胞诱导剂共培养3周。茜素红染色证实了间充质干细胞向成骨细胞分化的潜能。作者首次证明了短期动态压迫导致了中度的IVD退变。目前的研究结果被认为反映了IVD的早期退变，并揭示了与椎间盘退变相关的精确的机械转导途径。

We employed the co-culture method with iPS cells and IVD cells to differentiate iPS cells. At first, we directly co-cultured iPS cells with IVD cells. We induced embryoid body from iPS cells, in addition embryoid body cells were treated with retinoic acid for seven days. After treatment, mesenchymal stem like cells were differentiated.Furthermore these differentiated cells were co-cultured with osteoblast inducer reagent for three weeks. We have confirmed the potential of mesenchymal stem like cells to differentiate the osteoblast cell by Alizarin red staining. The authors, for the first time, demonstrated that short-term dynamic compression induced the moderate IVD degeneration. The results of current study were considered to reflect early IVD degeneration and revealed precise mechanotransduction pathways involved with intervertebral disc degeneration.