Development of muliticolor fluorescent assay based on nucleic acid-binding ligands

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A series of conjugates of abasic site-binding ligands with fluorescent signaling intercalators have been synthesized with a view toward gene analysis, especially single nucleotide polymorphisms (SNPs). With focusing on the variability of the emission wavelength of DNA-binding cyanine dyes by modification of their structures, blue, green, red fluorescence signaling conjugates have been developed for the selective detection of pyrimidine nucleobase over purine nucleobases.