Structural basis for specific cleavage of Lys63-linked polyubiquitin chains by Trabid

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We prepared the mouse, zebrafish and fly Trabid OTU domain and screened against 500 crystallization conditions. However, the OTU domains of Trabid were not crystallized. On the other hand, we determined the crystal structure of the HOIL-1L NZF domain in complex with linear di-ubiquitin. Together with structure based mutagenesis experiments our structure reveals the mechanism for specific recognition of linear chains by the HOL-1L NZF domain. This result was published in PNAS