Structural basis for specific cleavage of Lys63-linked polyubiquitin chains by Trabid

我们制备了小鼠、斑马鱼和苍蝇的Trabid OTU结构域，并针对500种结晶条件进行筛选。然而，Trabid的OTU结构域并未结晶。另一方面，我们确定了HOIL-1 L NZF结构域与线性双泛素复合物的晶体结构。结合基于结构的诱变实验，我们的结构揭示了HOL-1 L NZF结构域特异性识别线性链的机制。这一结果发表在PNAS上

We prepared the mouse, zebrafish and fly Trabid OTU domain and screened against 500 crystallization conditions. However, the OTU domains of Trabid were not crystallized. On the other hand, we determined the crystal structure of the HOIL-1L NZF domain in complex with linear di-ubiquitin. Together with structure based mutagenesis experiments our structure reveals the mechanism for specific recognition of linear chains by the HOL-1L NZF domain. This result was published in PNAS