Restraint stress induces connexin-43 translocation in rat heart

束缚应激诱导大鼠心脏连接蛋白 43 易位

• 批准号: 22890049
• 负责人: UNUMA Kana
• 金额: $ 2.01万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22890049/
• 关键词: 心臓性突然死; コネキシン; ギャップジャンクション; 情動ストレス; connexin43; α-アドレナリン受容体; 細胞間情報伝達; ストレス; アドレナリン受容体; miRNA

Immobilization(IMO) confers emotional stress in animals and humans. It was recently reported that IMO in rats induced translocation of connexin-43(Cx43) to gap junctions(GJs) and attenuated arrhythmogenesis with GJ inhibition. Few reports show the contribution of adrenoceptors to Cx43 upregulation in cardiomyocytes, but the involvement of adrenoceptors and ischemia in Cx43 translocation in IMO remains elusive. Male Sprague-Dawley rats underwent IMO and the ventricular distribution of Cx43 was examined by western blotting. IMO induced translocation of Cx43 to the GJ-enriched membrane fraction, with a peak at 60min. The IMO-induced Cx43 translocation was inhibited by pretreatment with theα-adrenoceptor. Theβ-blockers inhibited the premature ventricular contractions(PVCs) induced by IMO. Translocation of Cx43 to the GJ-enriched fraction occurs via theα-adrenoceptor pathway, independently of ischemia. Theβ-adrenoceptor pathway contributes to the inducing of PVCs in IMO

静止（IMO）会给动物和人类带来情绪压力。最近有报道称，大鼠的IMO诱导连接蛋白43（Cx43）易位至间隙连接（GJs），并通过GJ抑制减弱心律失常。很少有报道显示肾上腺素受体在心肌细胞中对Cx43上调的贡献，但肾上腺素受体和缺血在心肌组织中Cx43易位的参与仍然是未知的。雄性Sprague-Dawley大鼠进行IMO， western blotting检测Cx43在脑室的分布。IMO诱导Cx43向富含gj的膜部分易位，在60min时达到峰值。α-肾上腺素受体预处理可抑制imo诱导的Cx43易位。β受体阻滞剂可抑制IMO引起的室性早搏。Cx43向gj富集部分的易位是通过α-肾上腺素受体途径发生的，与缺血无关。β-肾上腺素受体途径参与了IMO中室性早搏的诱导

项目成果

Restraint stress induces connexin-43 translocation via a-adr enoceptors in rat heart

束缚应激通过大鼠心脏中的 a-adr 受体诱导 connexin-43 易位

• 发表时间: 2010
• 期刊: Circ J.
• 作者: Unuma K;Shintani-lshida K;Yahagi N;Tsushima K;Shimosawa T;Ueyama T;Yoshida K.
• 通讯作者: Yoshida K.

Connexin-43 Redistribution and Gap Junction Activation During Forced Restraint Protects Against Sudden Arrhythmic Death in Rats

强制约束期间 Connexin-43 重新分布和间隙连接激活可防止大鼠心律失常性猝死

• DOI: 10.1253/circj.cj-09-1019
• 发表时间: 2010
• 期刊: Circulation Journal
• 影响因子: 3.3
• 作者: Unuma K;Shintani-Ishida K;et al
• 通讯作者: et al

Restraint Stress Induces Connexin-43 Translocation via α-Adrenoceptors in Rat Heart

• DOI: 10.1253/circj.cj-10-0529
• 发表时间: 2010-12-01
• 期刊: CIRCULATION JOURNAL
• 影响因子: 3.3
• 作者: Unuma, Kana;Shintani-Ishida, Kaori;Yoshida, Ken-ichi
• 通讯作者: Yoshida, Ken-ichi

Connexin-43 redistribution and gap junction activation Buringforced restraint protects against sudden arrhythmic death in rats

Connexin-43 重新分布和间隙连接激活 Buringforced 约束可防止大鼠心律失常性猝死

• 发表时间: 2010
• 期刊: Circ J.
• 作者: Unuma K;Shintani-lshida K;Tsushima K;Shimosawa T;Ueyama T;Kuwahara M;Yoshida K.
• 通讯作者: Yoshida K.

硫化水素による心筋細胞障害の検討

硫化氢引起的心肌细胞损伤的研究

• 发表时间: 2010
• 作者: 白鳥典;船越丈司;鵜沼香奈;秋利彦;上村公一
• 通讯作者: 上村公一