Inhibition of RANK ligand-induced osteoclast differentiation by Porphyromonas gingivalis

牙龈卟啉单胞菌对 RANK 配体诱导的破骨细胞分化的抑制

• 批准号: 22890115
• 负责人: NAKAYAMA Masaaki
• 金额: $ 1.91万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22890115/
• 关键词: 歯周病原細菌; Porphyromonas gingivalis; 破骨細胞分化; 自然免疫; 抗酸菌; Toll-like receptor

In this study, we demonstrated whether P. gingivalis affects osteoclastogenesis induced by RANK/RANKL signaling. Osteoclastic precursor cells were challenged by P. gingivalis with RANKL at the same time, analyzed its differentiation for matured osteoclasts. Interestingly, the osteoclastic precursors were inhibited RANK/RANKL-induced osteoclastogenesis by infection of P. gingivalis. It was, however, contratory to my expectation that osteoclasts were promoted to form the large number of matured cells and activated bone resorption by P. gingivalis infection. According to investigate this inhibition by using precursor cells from knockout mice of TLRs and adaptor factors, MyD88 and TRIF, it revealed that innate immune response through TLR2/MyD88 signaling pathway were related with this inhibition.

在这项研究中，我们证明了牙龈卟啉单胞菌是否影响RANK/RANKL信号转导诱导的破骨细胞生成。同时用RANKL刺激成骨细胞前体细胞，分析其向成熟破骨细胞的分化。有趣的是，牙龈卟啉单胞菌感染抑制了RANK/RANKL诱导的破骨细胞生成。然而，牙龈卟啉单胞菌感染促进破骨细胞形成大量成熟细胞并激活骨吸收，这与我的预期相反。通过对TLRs基因敲除小鼠的前体细胞和衔接因子MyD 88、TRIF的研究，发现TLR 2/MyD 88信号通路介导的天然免疫应答与这种抑制作用有关。

项目成果

Helicobacter pylori VacA Reduces the Cellular Expression of STAT3 and Pro-survival Bcl-2 Family Proteins, Bcl-2 and Bcl-XL, Leading to Apoptosis in Gastric Epithelial Cells

• DOI: 10.1007/s10620-010-1420-1
• 发表时间: 2011-04-01
• 期刊: DIGESTIVE DISEASES AND SCIENCES
• 影响因子: 3.1
• 作者: Matsumoto, Ayako;Isomoto, Hajime;Kohno, Shigeru
• 通讯作者: Kohno, Shigeru

MicroRNA signatures in Helicobacter pylori-infected gastric mucosa

• DOI: 10.1002/ijc.25348
• 发表时间: 2011-01-15
• 期刊: INTERNATIONAL JOURNAL OF CANCER
• 影响因子: 6.4
• 作者: Matsushima, Kayoko;Isomoto, Hajime;Kohno, Shigeru
• 通讯作者: Kohno, Shigeru

歯周病原細菌Porphyromonas gingivalisの病原性発現に関する新しい分子伝達システム

一种表达牙周病原菌牙龈卟啉单胞菌致病性的新型分子传递系统

• 发表时间: 2010
• 作者: 北野千鶴;奥野裕子;梶谷優貴;古藤雄大;永井利三郎;中山真彰
• 通讯作者: 中山真彰

Streptomycin依存性結核菌における遺伝子変異の解明とStreptomycin耐性を誘導する新たな遺伝子変異

阐明链霉素依赖性结核分枝杆菌的基因突变以及诱导链霉素耐药性的新基因突变

• 发表时间: 2011
• 作者: 佐藤法仁;阿戸学;松村隆之;山崎利雄;関塚剛史;黒田誠;中山真彰;井上哲圭;本田尚子;土田耕三;小林和夫;大原直也
• 通讯作者: 大原直也

Identification of the novel mutations in 16S rRNA gene of the substrains of a streptomycin-dependent Mycobacterium tuberculosis Strain18b which confer streptomycin resistance in Mycobacterium

链霉素依赖性结核分枝杆菌菌株 18b 亚种 16S rRNA 基因新突变的鉴定，该突变赋予分枝杆菌链霉素抗性

• 发表时间: 2011
• 作者: Sato N.;Ato M.;Matsumura T.;Yamazaki T.;Sekizuka T.;Kuroda M.;Nakayama M.;Kobayashi K.;Ohara N
• 通讯作者: Ohara N