Development of bio-hybrid scaffold by using nanofibers and microenvironmental control of stem cells

在本研究中，我们试图控制间充质干细胞在各向异性构型的生物杂化支架上的分化，以开发用于干细胞培养的纳米纤维支架。我们关注具有各向异性的纳米纤维，因为生物组织的微环境是由具有排列的纳米纤维结构的细胞外基质组成的。我们使用了来自人骨髓的间充质干细胞(MSC)，因为它们具有造血支持活性。首先，我们研究了细胞分化对电纺法制备的各向异性纳米纤维的几何效应。结果发现，纤维的排列方式影响了MSC的分化。然后，我们通过覆盖各向异性纳米纤维支架在三维支架上培养细胞，将支架应用于高密度培养。因此，我们成功地构建了具有各向异性的厚层生物杂化支架。我们所创建的生物杂化支架有望应用于干细胞的高密度培养。

In this study, we have tried to controlof the differentiation of mesenchymal stem cells on the bio-hybridscaffold withanisotropic geometry to developnanofiber scaffold for the culture ofstem cells. We focused on nanofibers with anisotropy because the microenvironment of living tissues iscomposed of extracellular matrices which have aligned and nanometer-sized fibrous structures. We have used mesenchymal stem cells (MSC) derived from human bone marrow because they have hematopoietic support activity. First, we have examined the geometric effect of cell differentiation on anisotropic nanofibers fabricated by electrospinning method. As a result, wehave found that the fiber alignment affected the MSC differentiation. Then, we have cultured cells on the three-dimensional scaffold by overlaying the anisotropic nanofiber scaffold to apply the scaffold to high-density culture. As a result, we have successfully built thick bio-hybrid scaffold with anisotropy. The bio-hybrid scaffold that we have created was expected for the application of high denseculture of stem cells.