Development of treatment for bacterial translocation in rat's biliary atresia model.

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Male Wistar rats were divided into the three groups:Group1,control;Group2, receiving BDL only;Group3,receiving BDL and oral administration of TU-100.In Gr.2 and Gr.3,rats were sacrificed at 3,7 or 14 days after BDL. As a result:(1) Gr.3 had lower GPT on POD3 and lower serum HA on POD14 than Gr.1 and Gr.2,respectively(p<0.05).(2)BT was observed 0% in Gr.1 and 100% in Gr.2 on POD3,7 and 14. In contrast,it was detected 100% on POD3,20% on POD7, and 0% on POD14 in Gr.3. (3)Number and height of villus in Gr.3 were preserved compared to Gr.2,in time dependent manner(on POD 7,14, p<0.01).(4)Area of liver fibrosis and positive alpha-SMA area in Gr.3 were smaller than those in Gr.2(p<0.05).(5)In vitro experiment using HSCs isolated from BDL rats showed less expression of alpha SMA, timp1 and colI alpha 1 in TU-100 administration group. In conclusion,TU-100 may improve liver fibrosis by suppressing the activated HSCs and maintaining intestinal integrity in patients with biliary atresia.