Bone regeneration by gene activated matrix combined with nano-size calcium phosphate particle and minicircle DNA

利用微环DNA成功地制备了该载体，并发现该载体的细胞移植效率高于常规载体。然后结合绿色荧光蛋白基因制备了微环DNA/磷酸钙颗粒/胶原复合材料，并对材料进行了体内分析。在此期间，我们成功地制备了纳米亚微米磷酸钙颗粒，并报道了该材料的骨传导(K Miura，et al.应用表面科学.2013)。此外，我们还获得了骨再生材料的知识(A Matsui，et al.Cleft Palate-Craniofacl J，2013)。

By making use of minicircle DNA, this plasmid was sucsessfully made.And we found that cell transplantaiton efficiency of this plasmid was higher than conventional plasmid. After that we prepared minicircle DNA/calcium phosphate particle/collagen composite combined with Green Fluorescent Protein gene, and assessed the material in vivo analysis. During this period, we successfully made nano-submicro calcium phosphate particle and reported the osteoconduction of the material(K Miura,et al.Applied Surface Science.2013). Furthermore, we obtained the knowledge of bone regeneration material(A Matsui,et al.Cleft Palate-Craniofacl J,2013).