Body size evolution in Priapulida – ancestrally or secondarily small?

普里普利达的体型进化——先祖小还是继而小？

• 批准号: 454319699
• 负责人: Professor Dr. Andreas Schmidt-Rhaesa
• 金额: --
• 依托单位: Abteilung Wirbellose Tiere
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/454319699?language=en
• 关键词: Body; size; evolution; Priapulida; ancestrally

We are interested in changes of body size during evolution. The taxon Priapulida is ideal to investigate changes of body size, as among the only 21 described species are microscopic (2-3 mm) and macroscopic (several cm, up to 40 cm) species. With the ultimate goal to investigate genetic changes and drivers of evolutionary body size changes, we propose in this project to work out a robust phylogeny and compare morphological changes during ontogeny and between microscopic and macroscopic species. For a phylogeny (work package 1) we want to sequence transcriptomes of six species from the genera Priapulus, Priapulopsis, Halicryptus and Tubiluchus and complement these with data from other working groups on further species and genera (Meiopriapulus, Maccabeus). In three further work packages we will compare different organs (body cavity, musculature, cuticle, excretory organs, gonads) with different techniques (µCT, semithin and histological sectioning, phalloidin staining, tubulin staining). The work packages include comparison through the entire development of Priapulus caudatus, comparison between small (Tubiluchus) and large (Priapulus) species and intrageneric comparison of small (Halicryptus spinulosus) and large (H. higginsi) species.

我们对进化过程中体型的变化感兴趣。分类单元priapulida是研究体型变化的理想选择，因为仅有的21种物种是微观（2-3毫米）和宏观（几厘米，高达40 cm）的物种。凭借研究遗传变化和进化体大小变化的驱动因素的最终目标，我们建议该项目中的系统发育稳健，并比较在个体发育过程中以及显微镜和宏观物种之间的形态变化。对于系统发育（工作包1），我们希望对priapulus，priapulopsis，halicryptus和Tubiluchus的六种物种进行序列转录组，并与其他工作组的数据相辅相成（Meiopriapulus，MacCabeus，MacCabeus）。在另外三个工作包中，我们将比较不同的器官（体腔，肌肉，角质层，极端器官，性腺）与不同的技术（µCT，semithin和组织学切片，腓罗蛋白染色，微管蛋白染色）。工作包包括通过priapulus caudatus的整个发展，小（Tubiluchus）和大（Priapulus）物种之间的比较以及小（Halicryptus spinulosus）和大型（H. higginsi）物种的内部比较。