Architecture of the Central Nervous System in Nemathelminthes

线虫中枢神经系统的结构

• 批准号: 5453208
• 负责人: Professor Dr. Andreas Schmidt-Rhaesa
• 金额: --
• 依托单位: Abteilung Wirbellose Tiere
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2005
• 资助国家: 德国
• 起止时间: 2004-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5453208?language=en
• 关键词: Architecture; Central; Nervous; System; Nemathelminthes

The project wants to provide detailed information on the nervous system of representatives ofthe Nemathelminthes based on immunohistological staining techniques and confocal laser scanning microscopy. The first two phases produced basic information on the information content of single stainings in reference to the entire nervous system as well as methodological tools to work with animals having a cuticle. Data collection has been successful in three of the five taxa included into the project. 26 species of Gastrotricha were investigated, spanning the entire range of gastrotrich diversity. In the third project phase, few gaps (such as representatives of Lepidodasyidae) should be filled and the available data will be phylogenetically analysed. Two species of Priapulida were investigated and two further ones were collected, which will be investigated partly in the remaining time in the second phase of the project and partly in the third phase. Data for two kinorhynch species were obtained, with developmental data and a further species being scheduled for the third phase. Staining of nematomorph larvae was unsuccessful, but now controlled access to material is possible and the staining procedure will be optimized. In the third phase, adults will also be investigated. The collection of Loricifera was unsuccessful, but the collection attempt should be repeated. Within the third phase of the project, the collection and investigation of the species should be finalized. In parallel, joint analyses will be made. The groups working on nervous systems within the SPP are currently working on a glossary. In phase 3, a data matrix will be assembled and analyzed and morphological data are made available for an analysis together with molecular data gained within the SPP.

该项目希望通过免疫组织染色技术和共聚焦激光扫描显微镜技术，提供线虫类代表性动物神经系统的详细信息。前两个阶段产生了关于整个神经系统的单一染色的信息内容的基本信息，以及与具有角质层的动物一起工作的方法工具。在纳入项目的五个分类群中，有三个分类群的数据收集工作取得了成功。调查了26种腹毛类，涵盖了整个腹毛类多样性范围。在第三个项目阶段，应填补一些空白（如鳞屑科的代表），并将对现有数据进行遗传学分析。调查了两种Priapulida，并收集了另外两种Priapulida，部分将在项目第二阶段的剩余时间内进行调查，部分将在第三阶段进行调查。获得了两个kinorhynch物种的数据，发育数据和另一个物种被安排在第三阶段。线虫幼虫的染色不成功，但现在可以控制材料的获取，染色程序将得到优化。在第三阶段，还将对成年人进行调查。Loricifera的采集不成功，但应重复采集尝试。在该项目的第三阶段内，应最后完成物种的收集和调查。同时，将进行联合分析。SPP内的神经系统工作组目前正在编写术语表。在第3阶段，将组装和分析数据矩阵，并提供形态学数据和SPP中获得的分子数据进行分析。