Architecture of the Central Nervous System in Nemathelminthes

线虫中枢神经系统的结构

• 批准号: 5453208
• 负责人: Professor Dr. Andreas Schmidt-Rhaesa
• 金额: --
• 依托单位: Abteilung Wirbellose Tiere
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2005
• 资助国家: 德国
• 起止时间: 2004-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5453208?language=en
• 关键词: Architecture; Central; Nervous; System; Nemathelminthes

The project wants to provide detailed information on the nervous system of representatives ofthe Nemathelminthes based on immunohistological staining techniques and confocal laser scanning microscopy. The first two phases produced basic information on the information content of single stainings in reference to the entire nervous system as well as methodological tools to work with animals having a cuticle. Data collection has been successful in three of the five taxa included into the project. 26 species of Gastrotricha were investigated, spanning the entire range of gastrotrich diversity. In the third project phase, few gaps (such as representatives of Lepidodasyidae) should be filled and the available data will be phylogenetically analysed. Two species of Priapulida were investigated and two further ones were collected, which will be investigated partly in the remaining time in the second phase of the project and partly in the third phase. Data for two kinorhynch species were obtained, with developmental data and a further species being scheduled for the third phase. Staining of nematomorph larvae was unsuccessful, but now controlled access to material is possible and the staining procedure will be optimized. In the third phase, adults will also be investigated. The collection of Loricifera was unsuccessful, but the collection attempt should be repeated. Within the third phase of the project, the collection and investigation of the species should be finalized. In parallel, joint analyses will be made. The groups working on nervous systems within the SPP are currently working on a glossary. In phase 3, a data matrix will be assembled and analyzed and morphological data are made available for an analysis together with molecular data gained within the SPP.

该项目希望提供基于免疫组织学染色技术和激光扫描共聚焦显微镜的线虫代表神经系统的详细信息。前两个阶段产生了关于涉及整个神经系统的单一染色的信息内容的基本信息，以及处理有角质层的动物的方法学工具。该项目包括的五个分类群中有三个的数据收集工作取得了成功。调查了26种胃毛虫，涵盖了整个胃毛虫多样性的范围。在第三个项目阶段，应该填补一些空白(如鳞虫科的代表)，并将对现有数据进行系统发育分析。对两个种进行了调查，并采集了另外两个种，将在第二阶段的剩余时间和第三阶段的部分时间进行部分调查。获得了两个亲缘关系物种的数据，发育数据和另一个物种被安排在第三阶段。线虫幼虫的染色没有成功，但现在有可能控制材料的获取，染色程序将得到优化。在第三阶段，也将对成年人进行调查。Loricifera的收集失败，但应重新尝试收集。在该项目的第三阶段内，应最终完成对物种的收集和调查。同时，还将进行联合分析。SPP内从事神经系统工作的小组目前正在编制词汇表。在第三阶段，将汇编和分析数据矩阵，并将形态数据与在SPP中获得的分子数据一起用于分析。